Gall et al.: Geographic variation in population genetics of Oncorhynchus tshawytscha 



99 



Appendix A (continued) 



Alleles 



Alleles 



Appendix B 



Recently discovered allozyme variability 



Two monomeric mitochondrial loci of aconitate hydra- 

 tase, mAH-1 and mAH-4, are polymorphic in chinook 

 salmon. The rnAh-l(65) allele was observed primarily 

 in coastal California samples, although it is also pres- 

 ent in the Sacramento system. Three alleles at mAH-4 

 were important in differentiating coastal and inland 

 samples. Shaklee et al. (Wash. Dep. Fish., Olympia, 

 WA 98504, pers. commun., Feb 1991) have recently 

 performed breeding studies which confirmed the 

 Mendelian model of inheritance for these loci. 



Iditol dehydrogenase is coded by two loci in liver 

 tissue. The enzyme is a tetramer for which both loci 

 are assumed to be polymorphic. Variants were assigned 

 to a particular locus based on relative staining inten- 

 sities. The Iddh-l(O) allele was observed in Oregon and 

 coastal northern California populations. The Iddh-2(61) 

 allele was observed throughout the study area except 

 in samples from the Sacramento system, whereas the 

 Iddh-2(20) allele was only observed in the Sacramento 

 samples. 



Variation in NADP-dependent malate dehydrogenase 

 was expressed at two cytosolic loci using chinook 

 salmon muscle and heart tissue. MDHP-2 is also ex- 

 pressed in liver and eye tissue in juvenile fish. MDHP-1 

 variation has been described by Shaklee et al. (1990b). 

 Due to the low levels of variability found in the 

 Klamath-Trinity system, these MDHP loci wil be ex- 

 tremely important in the identification of fish from this 



area. The Mdhp-2(78) allele has nearly the same mobil- 

 ity as the Mdhp-l(lOO) allele, thus making identifica- 

 tion of heterozygous samples difficult. 



A duplicated and highly polymorphic monomeric 

 PGM locus was designated by two loci, PGM-3 and 

 PGM-4. These isoloci present particular difficulties 

 when estimating allele and genotypic frequencies 

 (Robin Waples and Paul Aebersold, NMFS Northwest 

 Fish Sci. Cent., Seattle, WA 98115, pers. commun., 

 June 1990). Six alleles have been identified in this 

 system and several individuals with three and four dif- 

 ferent alleles were observed. Therefore, standards are 

 required for correct analysis of banding patterns. 

 Similar expressions of variants are seen in both liver 

 and eye tissues. Conformance to Hardy-Weinberg pro- 

 portions at these loci has been found using goodness- 

 of-fit tests of expected and observed genotypes (Waples 

 and Aebersold, pers. commun.) and a protocol for 

 estimating allele frequencies from isoloci was presented 

 by Waples (1988). 



Triosphosphate isomerase is coded by four loci in 

 Chinook salmon. The products of TPI-1 and TPI-2 

 migrate cathodally, and those of TPI-3 and TPI-4 

 migrate anodally. Two variant alleles, Tpi-3(10A) and 

 Tpi-3(106), were observed from eye tissue, and TPI-4 

 variation has been described by Shaklee (pers. com- 

 mun.). Because Tpi-3(106) migrates close to Tpi-MlOO), 

 only fish homozygous for the Tpi-3(106) allele can be 



