Feeney: Post-yolksac development of two southern California sculpins 



455 



Orlhoiwpiits tnitcis - I | 



Isia San Geroriimo 



Puma Asuncion 



Pacific Ocean 



Figure 1 



Geographic range of Clinocottus analis and Orthonopias triads. 



contained an excellent series of both C. analis and 

 0. triads postflexion larvae, the discovery of which 

 became the impetus for the present study. 



The SWFSC material (10 specimens) is preserved in 

 5% formalin; some specimens (6) were collected in Baja 

 (6607-AX-110.32; 6806-JD-110.32; SWFSC/SIO H51- 

 106), the remainder were collected in California. Two 

 C. analis specimens (SWFSC/SIO H46-63) and two 

 0. triads (SWFSC 6607-AX-l 10.32) were cleared and 

 stained. 



CAS material included one lot of postflexion C. analis 

 (SU 68789, 70% ethanol), collected in Monterey Bay, 

 California. 



LACM specimens (fixed in 5% formalin and pre- 

 served in either 5% formalin or 70% ethanol) were col- 

 lected in coastal waters (<75m depth) of the Southern 

 California Bight between Pt. Conception and the Mex- 

 ican border. Most specimens were collected during the 

 Coastal Resources and 316b phases of the Ichthyo- 

 plankton Coastal and Harbor Studies (ICHS) Program 



and during the Bightwide Program; methods and 

 station locations can be found in Brewer et al. 

 (1981), Brewer and Smith (1982), and Lavenberg 

 et al. (1986). Also, five postflexion C. analis 

 specimens (LACM 45404-1, 45414-1-45417-1; in 

 ethanol) were collected at the Catalina Island 

 Marine Science Center (Ninos 1984). Six addi- 

 tional C. analis juveniles from the general LACM 

 collection were used: four collected at Santa Bar- 

 bara Island (LACM 31546-4), one at Catalina 

 Island (LACM 35695-1), and one at Palos Verdes 

 Peninsula (LACM 1993). 



Morphometric data, including preanal length, 

 body depth, pectoral length, head length, and eye 

 diameter were measured from 50 C. analis and 

 54 0. triads specimens. Data were entered into 

 an "Excel" spreadsheet program on a Macintosh 

 Ilci. Means and standard deviation of morpho- 

 metric measurements were computed using 

 "SYSTAT." Frequency plots of melanophores vs. 

 length were made using "SYGRAPH" and the 

 "LOWESS" (locally-weighted least squares) scat- 

 terplot smoothing method (Wilkinson 1989). 



Specimens were illustrated using a camera 

 lucida attached to a Wild M3 stereomicroscope. 

 Occurrence data are based on specimens taken 

 during 1978-85 on ICHS and Bightwide cruises 

 using a variety of sampling gears. During Coastal 

 Resources collections (ICHS cruises, 1978-79) 

 oblique bongo samples and discrete depth samples 

 were taken monthly along a grid of 10 transects, 

 each with 4 stations. The transects were evenly 

 spaced along the coast from Point Conception to 

 San Diego. The stations corresponded to bottom 

 depths of 8, 15, 22, and 36m. Additionally, 8 

 stations (4 sites each) were located in Los Angeles- 

 Long Beach Harbor and San Diego Bay. Integrated 

 water-column samples were collected by fishing a 70 cm 

 bongo sampler from the bottom to the surface. Discrete 

 depth samples were collected at the surface (manta 

 sampler), at the mid-depth of the water column (70 cm 

 bongo sampler) and at the bottom (70 cm bongo sampler 

 equipped with wheels). All samplers had nets of 335 ^i 

 mesh Nitex, and attached flowmeters gave estimates 

 of the volume of water filtered. During the 316b phase 

 (ICHS cruises, 1979-80) the number of transects was 

 increased to 20 and the number of stations was reduced 

 to 2 (8 and 22 m) except for 4 "expanded" transects 

 (Ormond Beach, Playa del Rey, Seal Beach, San 

 Onofre), which retained 4 stations (8, 15, 22, and 36m). 

 For epibenthic sampling, the benthic bongo sampler 

 was replaced by a larger "Auriga" sampler. Collections 

 were taken monthly during the 316b phase. Samples 

 were sporadically taken in 1981, but no data from them 

 are used here. 



