28 THE SAPROLEGNIACEAE 



Other cultures from No. io of August 25, 1912. 



In egg yolk broth only, in test tube. Abundant growth, dense at surface, delicate below. 

 Surface mat with a vast number of good sized gemmae of all shapes. A good many 

 oogonia (several hundred) with fine healthy eggs of usual size. Antheridia only one or 

 two to an oogonium, or seemingly absent from many. (Scarcity or absence of antheridia 

 made these very favorable oogonia for study. The wall is rather thin, and no pits 

 are to be seen.) 



On corn meal agar. Growth very strong and healthy. Many large gemmae of usual 

 varied shapes. Nothing else. 



In pea broth. Culture first made in test tube. After a month or more a transfer was made 

 to watch glass with new pea broth to see if the plant was alive. It was and grew well. 

 This last showed a great many rather small gemmae and no sexual reproduction or 

 sporangia. 



In egg yolk broth on ant. Infected by a bit of corn meal agar culture. Rapid and exten- 

 sive growtli throughout dish. Many gemmae and sporangia and a good many scatter- 

 ing oogonia of normal appearance with antheridia on each, but much fewer than usual. 

 Eggs of normal size and number, but in some cases not maturing. 



In Yi maltose peptone -j- yi egg yolk broth. Extensive growth of fine hyphae. No re- 

 production of any sort. 



On corn meal egg yolk agar. Growth luxuriant, covering dish. Immense number of gem- 

 mae. No oogonia. 



On ant in pea broth (50 peas boiled an hour or more in 500 c.c. of water) in sterile bottle. 

 It grew well and when examined a month later showed an extensive mass of vegetative 

 strands and a very few empty sporangia. 



Cultures from No. 9 of August 26, 19 12. 



In pea broth on yolk of egg, single spore culture. Growth strong and heavy. Numerous 



sporangia and large gemmae. No oogonia. 

 On corn meal agar, single spore culture. Growth very strong and healthy. Many large 



gemmae of usual varied shapes. Nothing else. 

 On yolk of egg in distilled water. Abundant sporangia, very abundant gemmae and a 



good many oogonia with many antheridia to each. 

 In maltose 5% + peptone .01%, single spore culture. Luxurious growth filling dish. 



A good many sporangia, some liberating spores, some not — in last case the contained 



spores often sprouted in position. A few small gemmae were formed. 

 In yi haemoglobin .05% + yi KN-PO, .1 % on ant (the culture was first grown to 



a good size in pure water, where many sporangia were formed, and then transferred to 



above solution). The sporangia did not empty and many gemmae formed. 



The following cultures were all made from a pure culture on 

 corn meal agar; the first six contained a .2% solution of the salt yi 

 and a .05% solution of haemoglobin ^ : 



In KNO3 -I- haemoglobin. Delicate growth of rather limited extent. Many cylindrical 

 sporangia, most of which retained the spores which sprouted by hyphae in position. 

 Some of the sporangia discharged a few spores, but most remained behind. In such 

 cases the remaing spores sent sprouts through the open tip as well as through the 

 sides. Gemmae very few and small. No sexual reproduction. 



In KHiPG, -I- haemoglobin. Delicate growth of rather limited extent. A good number of 

 gemmae of usual normal shapes. No sporangia and no sexual reproduction. 



