SAPROLEGNIA 29 



In NAHj04 + haemoglobin. Practically no growth. 



In Caj(PO.)- + haemoglobin. No growth. 



In K:S04 + haemoglobin. Delicate limited growth. Many normal sporangia, often 

 proliferating, a good many retained all or part of the spores, which did not sprout in 

 position. Gemmae rather few and simple. No se.xual reproduction. 



In Ca(N0j)2 + haemoglobin. Delicate, limited growth. .\ few sporangia, all retaining 

 the spores — no spores sprouting. .\ few scattered gemmae. No se.xual reproduction. 



In lactic acid, i drop to 50 c.c. HjO. No growth. 



In tannic acid, i drop to 10 c.c. H:0. No growth. 



The following experiments were made to test the best method of 

 preserving live cultures: 



Single spore culture (No. 10 of October 25, 1912) was put in vial of water in May, 1913, by 

 cutting out piece of corn meal agar on which it was growing. The vial was closed 

 with a plug of cotton and put in a dark place over summer. In December, 1913, tests 

 showed the culture dead, except for about yi the eggs, and they could not be made 

 to sprout. 



The same test was made for culture No. 5 of .April 19, 1913. and also a similar test using a 

 termite ant instead of a bit of agar. The first was found to be dead in December, the 

 latter alive. 



The same two tests were made for culture No. 2 of Ma>- 15, 1913, with exactly the same 

 results. 



Another test was made by putting a vigorous growth (single spore culture of No. 10 of 

 October 25, 1912) in an aquarium jar with algae on the laboratory table on March 7, 

 1913. No growth appeared on insects dropped in the jar September 18, 1917. 



2. Saprolegnia Kauffmaniana Pieters.* Bot. Gaz. 60:488, pi. 21. 1915. 



We have not found this, and the description is from Pieters, 

 omitting references to figures. The species is very near our 5. diclina, 

 of which it may be only a variety with larger eggs. For comparison 

 between this and 5. anisospora see under the latter. 



"This species was collected from algal material in the botanical 

 laboratory of the University of Michigan, of unknown source, but pre- 

 sumably from around Ann Arbor. 



"\'cgetative growth like that of 5. Jerax, with firm stiff hyphae; 

 sporangia freely produced and of the same size and appearance as in 

 5. ferax; gemmae round, oval, or irregular in shape, mostly single, some- 

 times in chains and freely produced; oogonia very large, on long or short 

 stalks, or intercalary; scattered; oval- or club-shaped, very rarely almost 

 round, the usual size being about 70-80X ioo-250,x. The smallest 

 oogonium noted was 30X70[x; oogonium wall thin and smooth, without 

 pits; oospores from 3 or 4 in small oogonia to very many in large ones, 

 averaging about 20-30 oospores per oogonium; oospores average about 

 30',i in diameter, contents granular without any conspicuous oil drop; 

 antheridia nearly always present, only occasionally absent on inter- 



* Dr. Pieters, who published this as S. Kaufmanniana, an incorrect spelling of Dr. 

 Kauflman's name, in reply to a letter, consents to a change to S. Kauffmaniana. 



