32 THE SAPROLEGXIACEAE 



In maltose + peptone alone. Growth good, filling dish. No sporangia or gemmae. 

 Many inflated oogonia, with normal antheridial threads of diclinous origin wrapped 

 about them, but no antheridia cut ofT; a good many of the oogonia with eggs in a 

 spherical or elliptic secondary oogonium inside and at the tip of an inflated and now 

 empty primary one, many without eggs (pi. 6, fig. 8). The wall of the inside oogonium 

 may be pitted and that of the outside bladder also if it is thick enough. (This experi- 

 ment repeated twice with single spore culture from No. 2 of January 6, 1913, again 

 twice with No. 4 of January 15, 1913; again with No. 6 of January 15, 1913, and 

 again with No. 6 of February 20, 1913 — in all cases with the same results). 

 On white of egg in distilled water. Growth delicate and short, but dense and healthy. 

 Very few if any sporangia. Abundant oogonia, with healthy eggs, averaging very 

 small. About J'a with only one egg. Antheridia on every oogonium. Where there 

 is only one egg there is usually only one antheridium, otherwise usually two or several. 

 Antheridial branches not quickly disappearing. 



On corn meal egg yolk agar. Luxuriant growth, filling dish. Immense number of oogonia 

 with one or more antheridia on each, all apparently of diclinous origin. The oogonia 

 were nearly all in a layer just below the surface of the agar; a few were borne above 

 the surface. 



On whole egg agar in distilled water. Oogonia terminal on main branches (generally), or 

 on long lateral branches which are two or more times (rarely only as long as) the diam- 

 eter of the oogonium. Antheridia on each oogonium, often there are both androgy- 

 nous and diclinous antheridia, nearly always some diclinous; androgynous also in 

 about yi cases. The number of androgynous antheridia varies a good deal in different 

 parts of the culture; they seem to be present where the growth is thickest ( No. 2 of 

 January 6, 1913). 



In equal parts of maltose and peptone and egg yolk broth. Growth extensive and delicate. 

 Many oogonia, nearly all inflated, many maturing good eggs, many not. Antheridial 

 threads very numerous, and apparently all diclinous. Antheridia not cut off, except 

 in a very few cases. No sporangia or gemmae. When eggs are formed they are cut 

 off in a special wall in the tip of a bladder, just as in maltose + peptone alone (single 

 spore culture from No. 2 of January 6, 1913). 



In corn meal egg yolk gelatin. Grew well and covered the dish, but after a few days liquified 

 the medium. No reproduction of any kind ( No. 4 of January 15, 1913). 



On whole egg agar in distilled water. Oogonia usually terminal on main threads; often 

 lateral on long threads, i. e., on stalks two or more times their diameter. Antheridia 

 on every oogonium, nearly always (only two exceptions seen) diclinous where the 

 oogonium terminates a main branch. In a great majority of cases (about 90%) also 

 diclinous where oogonia are on lateral branches. Short branches and bumps almost 

 entirely absent beneath the oogonia ( No. 6 of January 13, 1913). 

 The next three cultures were made with collection No. i of No- 

 vember 12, 191 7: 



On corn meal agar. Growth fairly vigorous, covering plate. Sporangia, containing spores 

 which remained in sporangia, not sprouting, fairly abundant. Many oogonia with 

 eggs. Antheridia present on about 90% of oogonia, mostly diclinous, but a fair 

 number of androgynous ones also. 



On corn meal agar cut from agar of preceding experiment in pure water without grub. Few 

 small sporangia formed and emptied on and around the edges of the agar. Good many 

 oogonia with eggs and androgynous and diclinous antheridia. Oogonia initials were 

 present when agar was cut and put in water. 



