SAPROLEGNIA 33 



On boiled corn grain. Growth vigorous, somewhat more curled and branched than on 

 grubs and flies. Sixirangia produced as nornuilly. Many oogonia with eggs; mostly 

 terminal on main hyphae. Fully 50% of oogonia had no antheridia. This culture 

 was repeated with the same results exce[n that about 75% of oogonia produced were 

 furnished with antheridia. 



The following experiments were to test the best method of preser\'- 

 ing live cultures: 



Single spore culture from No. 9 of November 25, 1912, put in aquarium jar with algae on 



March 3, iqi.v Was found to be dead when tested for life on September 18, 1917. 

 Single spore culture from No. 2 of January 6, 1913, put in an aquarium jar with algae on the 



laboratory table on March 7, 1913. Was found to be dead when tested for life on 



September 16, 1917. 

 Single spore culture of No. 6 of January 15, 1913, put in an aquarium jar with algae on the 



laboratory table on March 3, 1913. Was found to be dead when tested for life on 



September 16 1917. 

 Single spore culture from No. 9 of January 25, 19 1 2, put in vial on corn meal agar April 



10, 1913, and was found to be alive December i, 19 13. 

 Single s[X)re culture from No. 4 of January 15, 1913. put in vial on ant larvae April 10, 1913, 



and was found to be alive December l, 1913, and furnished growth from gemmae. 



All eggs had disorganized. 



4,. SaprolegniaanisosporadeBary. Bot. Zeit.46:6i9, pi. 9, fig. 4. 1888. 



Plates 7, 8, 9, and id. 



Main hyphae about 5-8 mm. long on a mushroom grub, of moderate 

 size at base, but quickly becoming smaller, the culture appearing quite 

 delicate in comparison with many other species; main hyphae from 

 40U. in diameter below to 1 1 ;j. or even less near the tip. Sporangia usually 

 borne on larger branches than the oogonia (but a good many oogonia 

 also borne on the larger branches), usually rather stocky and irregu- 

 lar and largest in the middle or near the base, sometimes regularly 

 tapering towards the end, very variable in size in the same culture, 

 about 8.6-i5.2yL, rarely up to i6.6;x thick, usually thicker than the strand 

 that bears them, often short and broad; proliferating as usual in Sapro- 

 legnia, or when in distilled water the greater part as in Achlya. Dictio- 

 sporangia have been several times observed. Spores remarkable in being 

 of two kinds, large and small and often intermediate sizes, usually' in 

 separate sporangia without constant regard to the size of the latter, a 

 single sporangium usually with spores of only one size, but occasionally 

 they are mixed; the smallest spores about 8-9;x in diameter, others 

 from 10. 5-1 1. 5j., the large ones from 13. 7-14. 8;j.; small and large spores 

 similar in structure, but the small are greatly in excess of the large 

 ones; in nearly all cultures there are formed in addition a few very 

 large spores at least twice the bulk of the ordinary large spores, these 

 appearing usually mi.xed with the latter. 



Oogonia numerous and formed in all ordinary culture media, borne 

 usually on the tips of long slender branches which arise from near the 

 substratum, often intercalary (very rarely two or three in a row), varying 

 to laterally sessile or on short or rather long lateral branches; typically 



