38 THE SAPROLEGNIACEAE 



On egg yolk in distilled water. Growth extensive. A few small gemmae. No sporangia, 

 apparently. This experiment was repeated and the growth was very strong. A good 

 many sporangia of usual shape. Very many gemmae of all shapes, most in chains — 

 some very long chain-forms, exactly as in 5. ferax. 

 In equal parts maltose 5% and peptone .01% solution. Growth very extensive, but 

 entirely sterile. No gemmae, sporangia or oogonia. Many threads were spirally 

 curled. 

 On corn meal egg yolk agar. Remarkable growth, different from any other water mold. 

 Agar was covered and an aerial growth followed that hid the agar under a dense pure 

 white cottony mantle }4 cm. thick, some fibers reaching still higher and touching the 

 cover. In the aerial mass were a good number of pear-shaped or nearly spherical 

 gemmae of smaller size than usual. Growth inside the agar only vegetative. 

 On corn meal agar. Fine growth filling dish. Many gemmae of usual shape. No oogonia. 

 In egg yolk broth. Growth good, but not over an inch in diameter. Many sporangia were 

 formed, but very few formed spores. Most of them became resting sporangia with 

 undifferentiated protoplasm. Many gemmae. These resting parts were left free by 

 the disappearance of the threads. All parts smaller than usual. 

 In egg yolk broth. Growth very strong and extensive. Many large gemmae and sporangia. 



No oogonia. Sporangia emptying normally. 

 On yolk of egg and ant in distilled water. Very strong growth, nearly filling dish. Many 



large gemmae. Also a good many oogonia were formed, but only near the ant. 

 On white of egg and ant in distilled water. Growth abundant, but more delicate than in 

 above. Many gemmae, smaller than above, and a good many sporangia. No oogonia. 

 On ants alone in distilled water. Growth strong, many sporangia and gemmae, about as 



in above culture. No oogonia. 

 On egg yolk in distilled water. Strong growth and many spores, both large and small. 



Many gemmae. No oogonia. Culture soon going to pieces. 

 On corn meal egg yolk agar. Grew well but did not send up any conspicuous growth in 

 air. Many oval, round, and pear-shaped gemmae formed. No other reproduction. 

 On mushroom grub in distilled water. Grew well. Many gemmae of all usual shapes and 

 many sporangia. All spores formed were small ones. A few \'ery large undivided 

 masses had been ejected as in most cultures. 

 On mushroom grub in distilled water. Two cultures were made. One showed apparently 

 no large spores, thousands of small ones. The other showed a good many large spores 

 also. Nearly all sporangia proliferated as in Achlya. Many gemmae in each as usual. 

 Two other cultures made a little later gave the same result except all spores small. 

 In 5% maltose in distilled water. Growth vigorous, but entirely vegetative. Threads 

 small. Later a fair number of spherical gemmae were formed. Another culture gave 

 the same result except that it went to pieces without forming any gemmae. 

 On mushroom grub in spring water. Growth and behavior as in distilled water except that 

 many sporangia proliferated repeatedly from within old ones, exactly as usual in 

 Saprokgnia. A second culture gave the same results. There were a few large 

 spores — not more than i in 100. Four years later (in 1918) repeated experiments 

 were made with No. i of April 4, 1918, to test again the effect of different water on 

 sporangia proliferation. The results were the same. In distilled water most of 

 the sporangia proliferate by lateral branching below as in Achlya, while in spring 

 water most of the proliferation is internal as usual in Saprolegnia. 



