SAPROI.Kr.XIA 39 



The cultures in the following solutions were all made on termites: 



In .1% KNOj. Cieniniao and sporangia present. Xo oogonia or anlluridia. 



In .1% KHjPOi. (irowth limited, and soon dying. 



In .1% Na»HPO(. Growth good. Not many sporangia, onl>- a iVw liberating spores. 

 A good many gemmae. 



In .1% of K;SOi. Growth good. Genmiae plentiful; sporangia few, some discharging, 

 some not. 



In .1% Caj(P04')5. Growth good. Many gemmae. Few sporangia; some not dis- 

 charging. 



In .1% Ca(NOs)s. Growth limited. Few gemmae. Xo sporangia. 



The following cultures were made in a solution consisting of equal 

 parts of a .2% solution of the salts indicated and a maltose-peptone 

 solution made by adding equal parts of 5% maltose and .01% peptone 

 solutions. Bacteria limited the growth in most cases. 



In Ca(XOj)i + maltose-peptone solution. Growth about J^ inch in diameter. Xo re- 

 production. 



In KXO3 -j- maltose-peptone solution. Same result as in preceding. 



In CajCPOj)" -|- maltose-peptone solution. Growth delicate and rather more e.xtensive 

 than in preceding. A few sporangia formed. A number of threads spirally wound. 



In KHiPO, -\- maltose-peptone solution. Delicate and extensive growth (2 inches in 

 diameter). Xo reproduction. 



In Xa^HPO) + maltose-peptone solution. Xo growth. 



In K2SO1 + maltose-peptone solution. Growth about I inch in diameter. A very few 

 gemmae. Xo other reproduction. 



In ^^2 maltose-peptone solution and li egg yolk broth. Dense growth of fine exen threads 

 l}^ inches in diameter. Xo reproduction. 



The following experiments were made to find the best method of 



preserving li\'e cultures: 



Culture put in vial on corn meal agar March l8, 191,1, was found to be dead, December I, 



I9«3- 

 Culture put in aquarium jar with algae, etc., on laboratory table on March t,, 1915. Xo 

 growth could be obtained from it when test was made September 18, 1917. 



The following cultures were made from No. 2 of January 2, 191 7: 

 On corn meal agar. Growth vigorous, covering the plate. A few sporangia, which did not 

 empty their spores. Many oogonia with eggs. Antheridia present in varying numbers 

 on every oogonium, all diclinous, so far as seen. This experiment was repeated several 

 times with var>ing results. A few sporangia were always formed, but often there 

 was no sign of any sexual reproduction. 

 In haemoglobin solution. Vigorous growth, but no eggs and no sporangia. Repeated this 

 experiment. Vigorous growth, .-\bundance of oogonia formed, but eggs went to 

 pieces as soon as formed. A few sporangia and some large and small s|iores given off 

 in early stage of growth. Antheridia present on all oogonia. 



