52 THE SAPROLEGNIACEAE 



above lO, rarely numerous" (see his figures 37-39, pi. 16). See under 

 S. monoica for note on the Michigan plant found by Pieters. There 

 seems no doubt that our Chapel Hill plant and Humphrey's plant are 

 the same as Tiesenhausen's var. glomerata, which he describes as follows 

 (translation) : 



"Turf delicate, up to i cm. broad. The side branches are thick- 

 ened, branched and contorted to form small knots, some of which are 

 sterile and mixed with similar ones bearing oogonia. Sporangia as 

 usual, with addition of conidia-like sporangia of various shapes, as pyri- 

 form or round. Oogonia on principal and side branches, 37-80[x thick, 

 stalks variously contorted and bent, often knee-shaped or knotted, 

 much branched, wall as a rule without pits, seldom with several. Eggs 

 22-24;x thick; 1-2 1, mostly 3-6 in an oogonium. Antheridia always 

 present, springing from the oogonial stalk or main thread or also from 

 nearby threads." Found in a small lake near Zermatt, Switzerland. 



Continuing the discussion the author brings out the fact that his 

 variety differs from S. monoica in the peculiar contorted clumps of side 

 branches and stalks and also in the larger eggs (io-22y. in 5. monoica 

 as he finds). Oogonial initials that are already provided with anther- 

 idia may halt and become sporangia. It is plain that this is our form 

 of S. monoica, the figures also agreeing well. It would appear from 

 Maurizio's description of his S. floccosa that it is very near if not the 

 same as the above (see p. 74). 



Rarely one or more very small eggs about half the normal size are 

 found in our plant mixed with the others, and oogonia have been observed 

 which contained only such subnormal eggs (fig. 10). Dwarfed spor- 

 angia with a single row of spores are met with, as are also dictiospor- 

 angia. Thick clusters of short, distorted branches that seem to be 

 antheridial branches are not rarely seen in some cultures without the 

 near presence of oogonia. This seems to be an abnormality induced by 

 the medium. In cases where the cylindrical oogonia are found inside 

 emptied sporangia there may be diclinous antheridia wrapped about the 

 sporangium, and at times one may enter the sporangium tip and run 

 down inside to the oogonium. Androgynous antheridia are usually also 

 present in such cases, running up inside from below. 



The following experiments were made with single spore cultures 

 from No. 7 of April 3, 1912: 



In equal parts of 5% maltose and .0T% peptone solution. Completely filled the petri 

 dish with very delicate threads. No gemmae, sporangia, or oogonia of good shape, 

 but a few bladdery initials were found which later sprouted by one or more tubes from 

 any point on the surface. This experiment was twice repeated with the same results, 

 except that there were more initials formed. The stalks of these bodies were often 

 twisted and curved. 



