5APR0LEGNIA 53 



In pea broth, February 28, 191;,. F.xtonsi\c growth. Large number of gemmae. Xo 

 sporangia or oogonia. The culture remained healthy and alive in this broth for about 

 two months. 



In corn meal agar. Grew vigorousK- and formed an immense number of oogonia. Most 

 were normal with good eggs and with antheridia. Many were inflated and no eggs 

 formed. Many gemmae were also present. This is the only Saprolegnia except 

 5. ferax that forms eggs in this medium, and the oogonia here are not so perfect as in 

 that species. Culture repeated twice with same results. 



In li corn meal + 'i egg yolk agar, March 4. lOiJ. X'igorous growth covering dish. .\n 

 immense number of good oogonia on rather longer stalks than usual. The stalks bear 

 at a little distance below the oogonium (about the middle) a forest of short lateral 

 threads, often branched, which may or may not reach the oogonium. When near 

 enough they will also apply themselves to other oogonia. This, so far, is the medium 

 that produces the most striking characters of this species. No gemmae. 



On whole egg agar, March 13, 1913. Strong growth. A good many sporangia. An im- 

 mense number of oogonia with average of 4-6 eggs. Antheridia on every oogonium, 

 generally several ; almost always some androgynous ones, and perhaps 2% of the oogonia 

 with diclinous ones in addition. Many short branches here and there like abortive 

 antheridia. 



On a bit of whole egg agar in distilled water. Oogonia nearly always on short lateral branches 

 (95Sc or more), occasionally terminating main hyphae (5% or less). Eggs generally 

 4, rarely 6. 



The following tests (single spore culture of No. 7 of Aprils, 1912) were 

 made to find the best way to preserve the life of cultures: 



(a) Two cultures were made January 13, 191 2, in sterile bottles containing pea broth 

 (50 peas boiled an hour or more in 500 cc. water). Growth was good, and when ex- 

 amined after a month showed results as follows: In pea broth alone, there was only 

 vegetative growth and all was dead; on ant larva in pea broth there were formed plenty 

 of oogonia and perfect eggs that were still alive, other parts dead, (b) In May, 191;,, 

 a culture was put in a vial of water by cutting out a piece of corn meal agar on which 

 it was growing. An ant lar\a was also dropped in at the same time. The vial was 

 closed with a plug of cotton and left in a dark place in the laboratory over summer. 

 A test for life was made in December, 1913, by dropping a mushroom grub in the vial, 

 and renewed grow^th resulted. The old eggs were alive (as shown by their normal 

 structure) both in the agar and on the ant that was put in at the same time with the 

 agar, but none had sprouted, (c) Culture was put in vial on corn meal agar March iS, 

 1913, and found to be dead December I, 1913. (d) A culture on an insect was put in an 

 aquarium jar with algae on March 3, 1913. Test for life was made in September, 1917, 

 by dropping in mushroom grubs but no growth appeared. 



9. Saprolegnia monoica var. vexans Pieters. Bot. Gaz. 60: 489. 1915. 



We have not found this, and the fcjUowing is taken from the original 

 description by Pieters: 



"This was secured from algal material collected at Sukey Lake, 

 near Ann Arbor, Michigan. The vegetative growth, sporangial char- 

 acters, and the formation and shape of gemmae do not differ in any 

 particular from those present in 5. monoica, S. ferax, or any other species 



