ACHLYA 105 



culture was put in fresh maltose-peptone to see if the bits of protoplasm were alive. 

 No Rrowtli resulted. 

 On corn meal, egg yolk agar. Cirowth very vigorous, soon covering the dish and many 

 long aerial hyphae reaching cover (li inch or more long). Very abundant oogonia 

 with hypogynal tube nearly always present, but not more than 5% with antheridial 

 cell cut ofT. Spines reduced to low prominences and angles in nearly all cases. No 

 other reproduction. 



The following six cultures were on lumps of egg yolk in distilled 

 water with the salt added: 



In .1% KNOj. Growth vigorous. No sporangia visible. Many oogonia, not more than 

 half maturing eggs. .\ good many intercalary. Sub-oogonial antheridial cell not 

 cut off in more than '3 cases, and no antheridial tubes seen. One case was seen of a 

 diclinous antheridium. 



In .1^0 KH.PO,. Growth vigorous. Many sporangia, discharging normally. Many 

 oogonia, only a small number maturing eggs, Antheridial cell cut oflf below in great 

 majority of cases, but no tubes formed. About 14 cases seen of outside antheridial 

 branches with club-shaped antheridia, nearly all diclinous. This shows the effect of 

 this salt in antheridial formation. 



In .1% NaHjPOt. Growth vigorous. A moderate number of s[5orangia, discharging 

 normally. Many oogonia, very few maturing eggs, and almost no antheridial cells. 



In .1% KiSO,. Growth vigorous. A few sporangia. Many oogonia and a good many 

 good eggs. Antheridial cells cut off in scarcely half the cases, but hypogynal tubes 

 present in most cases where there were antheridial cells. Several cases seen of outside 

 antheridial branches. 



In .i^c Ca3(POi)2. Growth vigorous. Many sporangia. In discharging about J 2 scatter 

 away from the tip with very obvious swimming motion, but slowly, and fall in an open 

 group. A drop of iodine on an emerging group of spores showed the cilia clearly at 

 the tip (pi. 29. fig. 4). Many oogonia, about one-third with good eggs, and these 

 mostly with antheridial cell cut off and with hypogynal tube. The most singular thing 

 about this culture is the occurrence frequently of rows of long eggs in sections of hyphae 

 which might be continuations of an oogonial cavity or might not. That is, long sec- 

 tions of the hyphae are cut off as oogonia and these are quite smooth, — not spiny like 

 ordinary oogonia. 



In 1% KsPO*. Slight growth. A good many inflated and abnormal oogonia without 

 eggs or antheridial cells. 



The following six cultures were made on lumps of egg agar in distilled 

 water with the medium added (infected from pure spore culture on 

 corn meal agar) : 



In .1% KNOj. Vigorous growth. Many oogonia, hardly half with antheridial cells, and 

 many of the latter with antheridial tubes. Out of more than 1000 examined 9 had 

 antheridia from outside, most of them androgynous. Oogonia normally spiny. .\o 

 sporangia to be seen. 



In .1% KHiPO.. Vigorous growth. Many normal sporangia and many normal oogonia; 

 antheridial cell and hyix)gynous tube nearly always present. At least 5% of the 

 oogonia with additional external antheridia which are either androgynous or diclinous. 



In .1% NaHiPO). Vigorous growth. Many normal sporangia. Three sporangia were 

 observed while discharging. The spores were killed on slide and all showed cilia very 

 plainly, both in and out of sporangium. Distinct swimming noticed before killing. 



