ACIILVA 115 



Four cultures on termite ants put in incubator at temperature of 36 C. produced many 

 oogonia and antheridia with coiled stalks; very few good eggs. 



On nutrient agar in one per cent solution of levulose (room temperature), llyphae 

 normal. A good many sporangia formed which emiilicd normally. \'ery few oogo- 

 nia and these smaller than usual and al)orli\ely shajied. Culture washed and trans- 

 ferred to pure water. .-X great ntany perfectly normal oogonia formetl with normal 

 eggs. 



On nutrient agar in ten per cent solution of levulose (room temperature). Hyphae normal. 

 A large number of sporangia formed, all of which emptied. Spores sprouted immed- 

 iately without coming out of cysts. .Many oogonia and about one-third of these with 

 eggs; the average number of eggs 3-4, a few oogonia with 8. 



8. Achlya proliferoides n. sp. 



Plate 36 



Growth moderately dense and strong, reaching a length of about 

 I cm. on a mushroom grub. Hyphae moderately branched, variable 

 in size, usually wavy and irregular, the tips hyaline and dying back 

 here and there as in A. imperfecta and A. flagellata. Sporangia sub- 

 cylindrical, usually bent, often with several openings; about 35-45;!. 

 thick as a rule, short or long, at times up to 14251A long. Spores ii-i2yL 

 thick, double ones not rare, often falling to the bottom in an open group 

 on emerging. Oogonia abundant, spherical, smooth, 40-55(1 in diam- 

 eter, racemosely borne on stalks that are about i-i ^^ times as long 

 as the diameter of the oogonia; wall hyaline, not thick; pits numerous 

 (usually), but not very conspicuous. Eggs eccentric, with a large oil 

 drop, about 18-24,1 in diameter, often elliptic, the great majority always 

 going to pieces before maturity on ordinary media. Antheridial branches 

 numerous, diclinous (inostly) or androgynous, usually long, contorted 

 and much-branched, in many cases coiling themselves about certain 

 selected hyphae which may or may not bear oogonia. Antheridia, one 

 or several, on every oogonium, elongated, applying their sides to the 

 oogonium or touching it by several blunt, foot-like processes. 



Not nearly so common in Chapel Hill as A. imperfecta or as A. 

 flagellata, but not rarely found with them, as in branch below Cobb's 

 Terrace (Xo. 3 of July 24, 1918. Type). 



As a rule very few and often none of the eggs mature in normal 

 cultures, such as on insects or corn grain in distilled water. They fall 

 to pieces into scattered granules or amorphous masses as soon as, or 

 before, a thin wall is formed. The oogonia often halt in their develop- 

 ment and send out a branch which bears another oogonium just as in 

 A. imperfecta. At times, however, instead of forming another oogon- 

 ium this branch may develop into an antheridial branch (fig. 3). Like 

 A. imperfecta again, the stalk of the oogonium may not rarely carry a 

 branch near its base which usually bears an oogonium. The tendency 

 to coiling in A. proliferoides is shown not only by the antiieridial branches 

 wrapping themselves about the hyphae, Ijut also by the frequent coiling 



