ACHLYA 135 



In maltose 5 % peptone .01 %. Oogonia in fairly large number, and reaching full size 

 with tubercles, but no antheridia and no eggs formed, contents going to pieces. 



On corn meal agar. Growth limited and purely vegetati\e. The threads became segment- 

 ed up into long sections, with dense jirotoplasm, as usual in the sjiecies. 



In equal parts of niallose-peptono and pea broth. Crowth good, about I inch in diameter, 

 dense and thick. A good many oogonia, and with normal eggs in most. Antheridia 

 on every oogonium, apparently — certainly on nearly all. 



On corn meal egg yolk agar. C.rowth slow, but extending over about a 2-inch circle, many 

 hyphae standing up into air ' s inch, but not dense. A consider.il)k' number of oogonia 

 with good eggs. Some had antheridia. 



In the following cultures equal parts of a 2% solution of the salts 



and of maltose-peptone solution (one part of 5% maltose+one part 



of .01% peptone) were used: 



In Ca(XOj)j + maltose-peptone solution. Strong, healthy vegetative growth, about i 

 nch in diameter. No reproduction of any kind. 



In KXOs -i- maltose-peptone solution. Growth as in preceding experiment. No re- 

 production. 



In Ca,fPO,). + maltose-peptone solution. Growth as in two preceding experiments, 

 except somewhat more delicate. No reproduction. 



In KH2PO4 -I- maltose-peptone solution. Most extensive growth of any in tiiis series. No 

 reproduction. 



In NaH-PO, + maltose- peptone solution. Growth short, contorted, and protoplasm seg- 

 regated into spots in larger hyphae. No reproduction. 



In KiSO, -I- maltose-peptone solution. Growth delicate, about I inch in diameter. No 

 reproduction. 

 Experiments to test the best method of preserving live cultures: 



Culture on corn meal agar put in vial of water, which was closed with a plug of cotton and 

 put in dark place in May, 1913. Test for life was made in December, 191,^ and it was 

 found to be alive (all old eggs were dead). 



Culture as above made March 17, 1913, was found to be dead on December i, 1913. 



Culture placed in aquarium jar with algae in laboratory on March 7, 1913. No growth 

 appeared when tested on September 18, 1917. 



21. Achlya dubia n. sp. 



Plate 40 



Main threads stout, little branched, only about 5 mm. long on a 

 termite, up to about 50 or 6oyi thick, tapering gradually, the rather 

 blunt tips clear and refractive while growing. Sporangia abundant, 

 terminating the main hyphae, long, only slightly thicker than the threads, 

 tapering a little towards the tip, increased sparingly by growth from 

 below as in Achlya; when mature discharging the spores as in Achlya 

 or as in Thrauslolheca in varying proportion, often about half and half 

 on a termite in sterilized well water, not rarely behaving as in Dicty- 

 tichiis. Spores normally al)out 1 1 .5;J. thick (but often much larger masses 

 of protoplasm arc found among them), encysting on emerging from the 

 sporangium and escaping for a swimming stage as in Achlya. Gemmae 

 abundant, formed by partitions in the threads l)ehind the sporangia, 



