138 THE SAPROLEGNIACEAE 



25 cc. levulose ^ + leucine -^ plus the following salts: 



(i) 10 drops 2% Mg SO, 

 (2) 10 drops 2% KXO3 

 13) 10 drop- 2% NaCl 



(4) 10 drops 2% Cas (PO^s 



(5) 10 drops 2% Ca (N03)2 



(6) 10 drops 2% Naj HPO, 



(7) 10 drops 2% KH2 PO4 



(8) 10 drops 2% K2 SO, 



Cochineal bugs were inoculated with the fungus and after ten days growth many of the 

 threads had become green in color. These green threads were otherwise perfectly normal 

 and in a healthy condition. The gemmae were almost uniformly red. 



Experiments to determine the resistance of the gemmae to extreme temperatures: 



(i) Resistance to cold. A culture with a number of resistant spores was placed on a block 

 of ice in the ice box and allowed to remain there over night. The following morning the 

 resistant spores were uninjured. 



(2) Resistance to heat. The same culture used in No. i was transferred to a petri dish 

 of hot agar which had just been melted in boiling water. The inoculated agar plate was 

 left out in room temperature and after two days growth had taken place to a length of i cm. 



EUROPEAN SPECIES NOT YET FOUND IN AMERICA 



Achlya spinosa deBary. Beitr. z. Alorph. u. Phys. der Pilze4: 54, pi. 

 4, figs. 13-18. 1881. 



The following is translated from the fuller description of 1888 (p. 

 647). 



"Main threads with many long interwoven side branches which 

 produce a woolly, snow-white turf which, if well nourished, may reach 

 a height of 2-3 cm. Reproductive organs sparingly produced. Zoo- 

 sporangia small, and producing few spores; often lacking. Oogonia 

 terminal, never intercalary [but see below], mostly barrel-shaped, densely 

 set with numerous, broadly conic, pointed or blunt projections, only 

 the upper and lower ends bare, the upper end with a conical point which 

 may be extended into a long beak. Eggs 1-2, rarely 3, in an oogonium; 

 of very various sizes, always about filling the oogonium; round or oval 

 when ripe with a large central fat globule and a circular or interrupted 

 row of peripheral globules [Korner]; without an obvious nuclear spot. 

 Antheridia about as often absent as present; in the latter case always 

 (?) only one on an oogonium; they are cylindrical-club-shaped and lay 

 one side on the oogonium, their short stalks springing from just below the 

 oogonial wall, rarely of diclinous origin. 



"Found once in the Titisee [lake] in the Black Forest, June, 1880." 



The above description does not agree with the original one in saying 

 that the oogonia are never intercalary, as the figures show several that are. 

 See remarks under A. glomerata. 



