535 



as before, after 11 to 12 hours, atid lasted about 4 hours, but the tem- 

 perature rose only to 39.0° C, or less thau that following the first 

 inoculation where 0.1 c. c. was used. This latter agrees with the observa- 

 tions of Dr. Koch, who explains the fact by the presence of more living 

 luberculons tissue at the beginning, so that a smaller amount of lymph 

 siifilees to produce a stronger reaction than later; through the injection 

 a small amount of the tissue capable of producing the reaction is used 

 up, and proportionally increasing doses are necessary to produce the 

 same degree of the reaction afterwards. 



As a control over the above experiment two healthy steers were 

 inoculated each with 0.3 c. c. of lymph. Careful observations every 

 hour revealed no increase in the temperature of the body. The 

 animals were slaughtered after 24 hours and the organs found to be all 

 in a normal, healthy condition. 



Experiments were made at the Veterinary School, in Berlin, by Eoeckl 

 and Schiitz {VerUffentUchungcn d. l: Gesundheitsamt, 1891, No. 5) with 

 two cows suspected of being tuberculous, and a healthy heifer. The 

 temperature, pulse, and general condition were closely observed for 

 several days prior to and following the inoculation. The animals were 

 all inoculated at the same time, each with 0.5 c. c. of lymph diluted with 

 4.5 c. c. of a one half per cent solution of carbolic acid in water. 



The fever reaction appeared in both the suspected cows after about 

 11 hours. The body temperature of one cow ranging from 38.8° to 

 390 rose to 40.3° C, and that of the other ranging from 38.1° to 38.7° 

 rose to 40.9° C. When slaughtered both cows were found to be infected. 

 No apparent reaction followed the inoculation of the heifer believed to 

 be healthy, and when slaughtered she was found to be in a healthy 

 condition. 



Anton Sticker, a veterinary surgeon in Cologne, reports [Arch./, ani- 

 malische Nahrungsmitielkunde, 1891, No. 4) a trial in which four "cows, 

 thought to be tuberculous, were each inoculated with 0.1 c. c. lymph. 

 Tlie fever reaction came in one after 7 hours, in the others after 9 hours. 

 < .'lie cow was slaughtered and found to be tuberculous. 



