288 TECHNIQUE 



Eosin.— To prepare this cytoplasmic stain, 1 gram of eosin powder 

 is added to 100 cc. of 70 per cent alcohol. This solution may be 

 diluted by an equal or greater volume of the 70 per cent alcohol, 

 if it is desirable to have the staining effected more slowly. 



Orange G and Erythrosin. — Both of these dyes may be prepared 

 in the same manner as eosin. A very satisfactory combination of 

 their effects may be obtained by mixing about one-third of erythrosin 

 with two-thirds of an orange G solution. So combined, sections 

 containing connective tissue and muscle have the former stained 

 orange and the latter a reddish color. 



REFERENCES. 



Anderson, J. 1933. How to Stain the Nervous System: A Laboratory 

 Handbook for Students and Technicians, Edinburgh, E. and S. Livingstone. 



Baker, J. R. 1933. Cytological Technique, London, Methuen & Co. 



Carleton, H. M. 1926. Histological Technique, London, Oxford Univ. Press. 



Conn, H. J. 1925. Biological Stains, Geneva, N. Y. 



Gage, H. S. 1925. The Microscope, Ithaca, N. Y., Cornstock Publ. Company. 



Galigher, a. E. 1934. The Essentials of Practical Microtechnique, Berkeley, 

 Calif., A. E. Galigher, Inc. Lab. of Microtechnique. 



Gatenby, J. B., and Cowdry, E. V. 1928. Lee's Microtomist's Vade- 

 mecum, 9th ed., London, J. and A. Churchill. 



Guyer, M. F. 1927. Animal Micrology, Chicago, 111., University of Chicago 

 Press. 



McClung, C. E. 1929. Handbook of Microscopical Technique, New York, 

 Paul B. Hoeber, Inc. 



