280 EXPERIMENT STATION RECORD. 



lesions; it is advisable to make cultures in suitable culture media, such as 

 glycerin potato, from the suspected material when Straus' method is employed ; 

 complement fixation is very reliable, but is probably too tedious and com- 

 plicated for routine procedure; cuti-reaction and ophthalmo reactions with 

 mallein have shown very poor results." 



[An atypical case of rabies], N. Foss (Vet. Rec, 2.'t (.1911), No. 1206, pp. 

 120, 121). — A description of a case in a 12-year-old pointer dog. 



The simplest m.ethod for staining the Negri bodies, M. Stutzeb (Ztschr. 

 Hyg. n. Infektionslcrank., 69 {1911), No. 1, pp. 25-28, fig. i).— The author has 

 modified Nicolle's method for detecting the Negri bodies as follows : 



The paraffin section is carried through xylol, alcohol, and water in the usual 

 manner, placed for from 5 to 15 minutes in Loffler's methylene blue solution 

 (which has been previously diluted with distilled water to make a transparent 

 solution), and differentiated with a 1 per cent solution of tannic acid. The 

 length of time for exposure to the solution depends upon the thicknesses of the 

 sections being stained. The progress of differentiation is observed by means 

 of the low power of the microscope until the nuclei of the nerve cells show up 

 plainly. 



When the differentiation is completed the preparation is taken from the 

 tannin solution and dried with filter paper, quickly carried through alcohol 

 and xylol, and mounted in Canada balsam. The Negri bodies appear reddish 

 violet, while the nerve cells are blue. 



Pyocyaneus infection in dog's and its similarity to rabies, W. F. Harvey, 

 R. M. Carter, and H. W. Acton {Vet. Rec, 24 {1911), No. 1202, pp. 57-59).— 

 In this paper the results of examining 5 cases in dogs are reported, which in 

 one way or another would lead one to suspect rabies. In all instances 

 Bacillus pyocyaneus was detected and could be recovered from guinea pigs 

 and rabbits which were inoculated with the brain substance and the blood. 

 Negri bodies were not noted. 



A paratyphoid-like bacillus isolated, from a dog, E. H. Rxtediger {Jour. 

 Infect. Diseases, 8 {1911), No. .'/, pp. //S6--'/99). — During a routine test for Negri 

 bodies in a dog the author came upon a case which was negative as regards 

 Negri bodies, but from the blood of the heart and the pus from the lung a small 

 rod-like bacterium was isolated which resembled the paratyphoid bacillus 

 morphologically and biologically. . It was found to be highly pathogenic for 

 guinea pigs, rabbits, and monkeys, and had a tendency to produce pneumonia. 

 A soluble toxin, according to the author, is probably not produced by this 

 organism. 



While comparing this organism with strains of paratyphoid A and B the 

 author incidentally noted that practically no difference exists between the 

 bacilli usually classified as A and B. 



Susceptibility of certain domestic animals to plague infection with par- 

 ticular reference to that of ground squirrel origin, G. W. McCoy and C. W. 

 Chapin {Jour. Infect. Diseases, 9 {1911), No. 3, pp. 276-281).— "Although 

 there are a few discordant reports, the general experience with animals other 

 than rodents and anthropoids seems to be that local and temporary constitu- 

 tional effects are observed in a considerable number of cases after feeding or 

 after subcutaneous inoculation with cultures of Bacillus pestis or with plague 

 tissues, but that fatal infections are extremely rare. The cat is an exception 

 to this rule, and exhibits a considerable degree of susceptibility. The results 

 of our experiments have been quite in harmony with the above generalization. 

 B. pestis was demonstrated at the site of inoculation after several days in the 

 case of the calf, hog, and sheep, but not in the case of the goat. Adequate 



