VETEKINARY MEDICINE. 679 



were made to scrape off the culture on the surface. Moist chalk and moist clay 

 were found to be much better than gypsum rods. 



Diagnosis of glanders with the precipitin reaction and the relation of 

 these precipitins to the glanders agglutinins, M. Mijller {Ztschr. Immuni- 

 tdisf. u. Expt. Thcr., I, Orig., 3 {190'.)), A'o. //, pp. 401-^21, fig. 1; ahs. in Hyg. 

 Rundschau, 21 (1911), No. 8, pp. J/JjO, J/.'/l). — The precipitin method can be used 

 for the diagnosis of glanders provided the various specifications prescribed by 

 the author are observed. In guinea pigs the precipitin content of the blood first 

 increases and then the agglutinin content. The reverse is the case with the 

 rabbit. The layer test is to be given the preference over the mixing test. For 

 the precipitin reaction filtrates from bacterial emulsions which were not pre- 

 viously shaken are advised. 



Kapid diagnosis of glanders with the aid of the complement fixation 

 method, Miessner {Centhl. Bakt. [etc.], 1. AM., Orig., 60 {1911), No. 3-1,, pp. 

 327-329). — After pointing out that he had already devised a rapid, agglutina- 

 tion method, the author states that by the use of a water bath instead of a 

 thermostat regulated to 37° C. for the complement fixation method judgment 

 can be passed within an hour as to whether the horse is glanderous or not. 



Rapid diagnosis of glanders, M. MiJLLER {Centhl. Bakt. [etc.], 1. AM., Orig., 

 61 {1912), No. 7, pp. 607, 60S). — A polemic in regard to priority in the use of 

 the agglutination-centrifuging method " for diagnosing glanders. 



Microscopic diagnosis of rabies, S. B. Moon (Jour. Amer. Med. Assoc., 57 

 (1911), No. 9, p. 735). — A modification of the Van Gieson technique, which is 

 employed at the Pasteur Institute of Virginia, and has furnished good results, 

 is as follows: 



" Smears containing pyramidal cells of the hippocampus, or Purkinje cells of 

 the cerebellum, are prepared in the usual manner, allowed to dry, and fixed for 

 2 minutes in ethyl or methyl alcohol. They are then dried with blotting paper 

 and stained. Two or 3 drops of a saturated solution of rosauilin in acetone 

 (U. S. P.) are added to 10 cc. of water, which is agitated with a pipette, and 

 soon becomes a brilliant rose-red color. Then 2 drops of a half-saturated 

 aqueous solution of methylene blue are added, and the satin is applied to the 

 smears. Staining is completed in 1 or 2 minutes, without heat. The slide is 

 then washed under the tap, dried, and examined. Should a weaker than 

 saturated solution of rosauilin be employed, it is only necessary to add more 

 of it, drop by drop, to the water, till the usual brilliant rose-red tint is ob- 

 tained, when the result will be found about the same as that obtained with the 

 saturated solution." 



The essential features of the lesions caused by Trypanosoma hippicum, 

 S. T. Darling (Compt. Rend. 8oc. Biol. [Paris], 72 (1912), No. 5, iip. 150- 

 152). — The author has studied lesions in mules and horses, monkeys (Cebus 

 and Nyctipithecus), coati (Nastia narica), raccoon, dog, rabbit, guinea pig, rat, 

 and mouse, and with 'the exception of Nyctipithecus and the raccoon com- 

 parisons have been made with normal tissues. " The lesions, similar in type 

 to those caused by other pathogenic tryiianosomes, are due to an intoxication 

 resulting in cellular degenerations, anemia, lymphocytosis, terminal ecchymoses 

 of serous and mucous membranes, edema and extensive hyperplasia of lymphoid 

 and myeloid tissue, with focal, cellular infiltrations which are partly inflam- 

 matory and partly hemopoietic in function." 



A method for the bacteriological detection of tuberculosis in bovines, 

 E. ScHARR and Opalka (Berlin. Ticrdrztl. WchnsGhr., 27 (1911). No. 46, pp. 

 833-80, figs. 5). — The method consists of swabbing the walls of the trachea of 



a Berlin. Tieriirztl. Wchnschr., 1908, No. 34, pp. 595, 596. 



