782 EXPERIMENT STATION RECORD. 



(livisicm into 2 such bodies, aud so on. Tlie smaller cocci could be made to 

 pass through a Berkefeld filter and a colloidal filter. 



The treatment of foot-and-mouth, disease by Doyen's method, G. Moussu 

 (Rec. MM. V^t., 88 (1911), No. 17, pp. 533-5 il).— A critical discussion in 

 regard to this method and its origin. The results obtained with it were 

 unfaAorable. 



The probable influence of the open water trough in the spread of glanders, 

 A. T. Kinsley iAiiicr. Vet. Rec, JiU (I'JJJ), Xo. 5, pp. Ii3.i-(i.is). — A paper pre- 

 sented at the fifteenth annual meeting of the United States Live Stock Sanitary 

 Association, held at Chicago in December, 1911. 



The incidence of Mediterranean fever in Malta and its relationship to the 

 size of the goat population, J. W. H. Eyre (Lancet [London], 1912, I, No. 2, 

 pp. 88, 89, figs. 3). — It is pointed out that the main causative factor in reducing 

 the prevalence of Malta fever on the Island of Malta w^as the destruction of 

 large numbers of milch goats, many of which produced milk containing Micro- 

 cnrciis melitcnsiii. The goat population of Malta was reduced from 17.110 in 

 1907 to 7,619 in 1910. 



About the sero-diagnosis of Malta fever, Rouslacroix (Compt. Rend. Soc. 

 Biol. [Paris], 10 (1911), No. 10, pp. 397-399).— The results .show that in most 

 instances where a serum specific for the Micrococcus mclitensis was employed 

 no agglutination reaction with the Bacillus typhosus was obtained. In only 

 1 out of 56 cases (1.84 per cent) did the serum agglutinate positively for 

 both organisms. The dilution of the specific serum used was 1 : 50. In cases 

 where a double reaction is obtained an infection with both organisms must be 

 suspected. 



Piroplasms of zebus and of their crosses in Tunis, W. L. Yakimoff and 

 Nina Kohl-Yakimoff (Bui. 8oc. Path. E.rot., 7/ (1911), No. 7, pp. 451, .',52).— 

 The authors have examined the blood of 2 zebus, of 12 zebu crosses with native 

 cows, and of a cross with a French cow, and found piroplasms to be present in 

 the blood of both the zebus and in 10 of the 13 hybrids. The piroplasms ob- 

 served appear to belong to a single species, either Piroplasma bacilliformis or 

 P. parva. In no instance was P. bigcminum discovered. Whether or not the 

 organism is pathogenic in these animals was not determined. Attention is 

 called to the double importance obtaining, first, in the zebu and zebu hybrids 

 furnishing a reservoir for the virus in its transmission to native stock and 

 that imported from France, and. sec-ond, in the lowered resistance of the zebus 

 and their crosses to other infectious di.seases. 



Immunizing against rabies, C. Fermi (Centbl. Bakt. [etc.], 1. Abt., Orig., 

 61 (1912), No. 7, pp. .^)96, 597). — The method utilized consistetl of giving normal 

 and rabid nerve substances i>er mouth. All animals were found to be immune 

 upon feeding for a period of 2 months. This was determined by subcutaneous 

 infection with the street and fixed virus. No deaths resulted. 



An antirabic vaccine, D. Semple (Brit. Med. Jour., 1911, No. 26^2, p. 392; 

 abs. in Vet. Rec, ,?'/ (1911). No. 1209. p. 171). — As the methods commonly used 

 for preparing antirabic vaccine are somewhat unsatisfactory, the author recom- 

 niends making " an 8 per cent clilution of rabies virus in normal saline, to which 

 1 per cent carbolic acid is added, and to keep this at a temperature of 37° C. for 

 24 hours. By this means the virus is killed. The mixture diluted with an 

 equal bulk of saline solution gives a vaccine containing 4 per cent virus and 

 0.5 per cent carbolic acid. This was found to confer a high degree of immunity 

 on monkeys, dogs, and rabbits, and the serum obtained from these animals had 

 a well-markeil rabicidal action on living virulent virus." 



The action of formaldehyde upon tetanus toxin and other bacterial toxins, 

 M. VON EisrEB and E. Lowenstein (Centbl. Bait, [etc.], 1. Abt., Orig.. 61 (1911), 



