784 EXPERIMENT STATION RECORD. 



sliuws no (lotinite growth on fresh bovine tissue or fresh extracts of bovine 

 tissue removed aseptically and placed into sterile tubes. There is no evidence 

 that Johne's bacillus grows in symbiosis with an ultra-microscopic virus. 



"The specific bacillus will grow on media containing the dried and powdereil 

 growth of certain acid-fast bacilli which have been previously killed, and this 

 is so even when the dead bacilli have been kept for a period of 8 years, and 

 subjected to a temperature of 115° C. in tlie autoclave for 1 hour. The most 

 suitable bacillus to incorporate in the medium is the timothy-grass bacillus, 

 and to a somewhat less degree the smegma bacillus of Moeller and the nasen- 

 schleim bacillus of Karlinski. The human type of tubercle bacillus is also good, 

 but on media containing the avian type Johne's bacillus grows very slightly, 

 if at all. With the few bovine strains tested in media we were unable to get 

 any definite evidence of growth with Johne's biicillus. Tubercle bacilli isolated 

 from cats also gave negative results. The essential substance or substances 

 necessary for the growth of Johne's bacillus can be extracted from the various 

 acid-fast bacilli which give positive results by means of hot ethyl alcohol. We 

 have isolated Johne's bacillus from consecutive cases of pseudo-tuberculous 

 enteritis, and have proved the moiThological and biological characters of the 

 bacilli isolated to be identical in every respect. . . . 



" The specific bacillus, when inoculated intravenously or given by the mouth 

 to bovines, reproduces pseudo-tuberculous enteritis in the animal, and this can 

 not be distinguished from the original disease either clinically during life or 

 post-mortem. Further, the bacillus can be recovered from the lesions in the 

 intestine of the inoculated animal, and shows characters in every way iden- 

 tical with the bacilli isolated fi*om the original cases. 



"Animals suffering from pseudo-tuberculous enteritis, either normally con- 

 tracted or experimentally produced by the inoculation of pure cultures of 

 Johne's bacillus, give no definite reaction with diagnostic vaccines prepared 

 from cultures of the timothy-grass bacillus or from the avian tubercle bacillus. 

 Vaccines can be prepared from cultures of Johne's bacillus similar to those 

 prepared from other acid-fast bacilli. Diagnostic vaccines prepared from cul- 

 tures of Johne's bacillus grown on tubercle bacillus medium gave positive reac- 

 tions with tubercular animals, which proved the medium used to be unsuitable for 

 the preparation of a s]jecific diagnostic vaccine for pseudo-tuberculous enteritis. 

 Vaccines prepared from cultures of Johne's bacillus on a timothy-grass bacil- 

 lus medium gave negative reactions with normal and with tubercular animals, 

 and also with bovines suffering from pseudo-tubercular enteritis. We believe 

 this to be due, partly to the small amount of growth in the fluid media, and 

 partly to the fact that most of the growth was obtained from solid media 

 and therefore not made in the same manner as diagnostic tuberculin. We 

 also believe that a highly concentrated vaccine will be required, and that we 

 shall be able to prepare this now that one of our strains of Johne's bacillus has 

 started to grow on the surface of fluid media containing the timothy-grass 

 bacillus." 



A biblography of 4.2 titles is appendetl. 



Infectious abortion of bovines, ZwicK (Deut. Tiemrztl. Wchn^cJu:, 19 

 {1911), l\'o. 51, pp. 181-78.'}: abx. in MiiDchcii. Tirrarzil. Wchm^cJir., 56 (1912), 

 No. 2, pp. 36-88). — Previously noted from another source (E. S. R., 24, p. 785). 



A flagellated organism encountered in a vulvo-vaginal pustulo-ulcerous 

 eruption in a buffalo, I. Poenaru {Compt. Riind. Soc. Biol. [Paris], 70 {1911), 

 No. 15, pp. 621,, 625; Vet. Rec, 21, (1911). No. 1215, p. 2.',6).—A microscopical 

 examioation of the scrapings from ulcers obtained from a case of pustulo- 

 ulcerous inflammation of the vagina, which later involved the urethra and the 

 bladder, revealed the presence of an organism, among many others, which " had 



