184 EXPERIMENT STATION RECORD. 



p^ 57), — By innmiiiizins rabbits against streptococcic infection the author found 

 that the bacteria for preparing the vaccines which were liilled with urea or 

 galactose sohition were more efficient than those killed by heat. It was found 

 that one strain of the vaccine bacteria is antagonistic to other strains of 

 bacteria. A monovalent serum protects in the same manner. An antistrepto- 

 coccic serum prejiared from streptococci obtained from cases not having 

 strangles was efficient for strangles. The serum from horses which recovered 

 from strangles contained practically no protective bodies. 



Protective and curative vaccination against tetanus, Schuemacher {Mitt. 

 Ver. Bad. Tiemrzte, 10 (.1910), No. 5, i)p. 75, 76; abs. in Berlin, Tierarztl. 

 WchnscJir., 27 (1911), No. 6, p. 105). — Many cases of tetanus occurred in unvac- 

 cinated horses, whereas animals vaccinated biyearly with 10 cc. of Pasteur's 

 antitetanic serum never took the disease. Two cases of severe tetanus were 

 treated subcutaneously and successfully with 50 cc. of this serum on the 

 first day, 30 cc. on the second day, and 20 cc. on the third day. Another animal 

 which was treated at a late hour died. 



In regard to tuberculosis infection and its virus, A. Fontes (Mem. Inst. 

 Oswaldo Cruz, 2 (1910), No. 1, i)p. 1 J/ 1-1 Jf6).— As a result of his work with 

 filtered tuberculous pus the author considers the granulations in the tubercle 

 bacillus the living and infective factor of the organism. It is further stated 

 that certain typical clinical cases which yield negative results for the tubercle 

 bacillus with the usual staining methods can be explained by the presence of 

 these granulations in the body, as the granulations can eventually develop into 

 tubercle bacilli. 



In regard to the biology of the tubercle bacillus, H. Aronson (Berlin. 

 KUih. Wchnschr., J,7 (1910), No. 35, pp. 1617-1620; ahs. in Chcm. Zentbl., 1910, 

 II, No. 19, pp. 1^91, l/f92) .^The specific staining power of the tubercle bacillus 

 is due to the presence of a wax, which, according to the author, contains an 

 alcohol which is not cholesterin. The wax can be absolutely extracted with 

 trichlorethylen, which at the same time extracts Much's granulse. According 

 to this finding the latter can not consist of a particular form of protein. The 

 solvent action of neurin for tubercle bacilli, according to the author, is due to 

 strongly alkaline properties which the bodies have, which belong to the neurin 

 class. The solution can also be made with tetramethyl ammonium hj'drate. 

 See also a note by Lindeman (E. S. R.. 24, p. 2S3). 



A new method for the cultural and microscopic detection of the tubercle 

 bacillus in sputum and other tuberculous material, Uhlenhuth and Kersten 

 (Ztschr. Expt. Path. u. Titer., 6 (1909), No. 3, pp. 759-776).— The method con- 

 sists of placing the sputum in a 15 per cent antiformin solution in a large sterile 

 Drigalski dish which sits on a dark background. After the sputum has be- 

 come liquefied the I'emaining flocculent masses are fished out with a platinum 

 loop and placed in a physiological salt solution to remove the adherent anti- 

 formin. The mass is then carefully rubbed up with solidified glycerin bovine 

 serum (6 to 8 tubes), the cotton plugs of the tubes fiamed, and sealed with 

 paraffin. 



The method furnishes very good results. 



Inhalation and feeding tests with tubercle bacilli and bovines to deter- 

 mine the smallest possible amount necessary for infection, A. Weber and 

 C. TiTZE (Tube)k.ulose Arh. K. Gsndhtsamt., 1910, No. 10, pp. 146-156).— The 

 tests showed that at least 0.00001 gm. by inhalation and 0.01 gm. per mouth of 

 tubercle bacilli are necessary to infect bovines. 



Open-air experiments with tuberculous cattle, J. G. Rutherford (Amer. 

 Vet. Rev., 38 (1910), No. 3, pp. 31 3-3 J,9).— The author, after slaughtering all 

 clinical cases of tuberculosis in a herd, observed the 42 survivors for some 



