610 EXPERIMENT STATION EECORD. 



Bulgarian ferment was found to attack casein to only a very small degree. In 

 this respect the author's findings do not agree with those of Effront noted above, 

 and the author believes that the latter had a contamination with some foreign 

 organism. 



In regard to the Bulgarian ferment, J. Effbont (Compt. Rend. Acad. Sci. 

 [Paris], 152 (1911), 2so. 8, pp. ^6S-J,65; ahs. in Centhl. Balct. [etc.], 2. AM., SO 

 {1911), No. 19-20, pp. 501, 502).— The author has continued his work in this 

 direction, and now reports the results with Bertrand's Bulgarian ferment and 

 Jaourt's ferment (Lactobacilline, Maya bulgare. and Maya nutricia). All the 

 latter preparations mentioned converted milk to a yellow fluid, while Bertrand's 

 ferment produced a jelly-like coagulum. 



The results in detail were as follows: The percentage of nitrogen in the form 

 of amids was 16.2 for Bertrand's Bulgarian ferment and from 71 to 87 for 

 Jaourt's artificial preparation, the total acidity expressed as tenth-normal alkali 

 in 100 cc. solution was 280 cc. for Bertrand's ferment and from 78 to 110 for 

 Jaourt's ; and the volatile acid expressed as tenth-normal alkali per 100 cc, was 

 18 cc. for Bertrand's ferment and from 55 to 88 for Jaourt's. 



Contributions to the biology of colostrum, J. Bauee (Deut. Med. Wchnschr., 

 85 {190D), yo. 38, pp. 1657-1659; ahs. in Hyg. Rundschau, 20 {1910), No. 11. pp. 

 616, 617). — An antiserum was prepared with which it was possible, with the aid 

 of the complement binding method, to differentiate colostrum from cow's milk. 

 The antiserum deviated with the same colostrum in dilutions up to 1 : 100,000; 

 but with cow's milk and bovine serum it was only specific in dilutions of 

 1 : 10,000. The colostrum of the first day evidently contains bodies which act 

 as antigens and which are also contained in the blood serum but not in the 

 milk of the same type of animal. First-day colostrum is also hemolytic for 

 guinea-pig corpuscles, while milk is not. 



The relation between milk, colostrum, and serum of the cow, F. Graetz 

 (Ztschr. Imnuuiitdtsf. u. Expt. Ther., I, Orig., 9 {1911), No. 5, pp. 677-708; 

 abs. in Chem. Ahs., 5 {1911), No. 15, 2500). — It was determined with the pre^ 

 cipitin, anaphylaxis, and complement-fixation tests that there is only a quanti- 

 tative relation between these .3 fluids and not a qualitative one. Colostrum 

 takes the intermediate position. 



Estimating the fat in milk by the Eose-Gottlieb method, C. Hutge (Rev. 

 Gin. Lait, 7 {1909), No. 12, pp. 265-272; ahs. in Molk. Ztg. [Eildesheim], 24 

 {1910), No. 13, p. 218). — Tests were conducted with Rohrig's apparatus, and 

 the results show that the upper ethereal layers contain more fat than the lower 

 ones. The author claims that better results may be obtained if the glass cock 

 of the apparatus is placed lower down and the whole ethereal layer is used for 

 the determination. Two washings are made with ether after drawing off the 

 fluid. (In this regard see also a previous note (E. S. R.. 23, p. 217).) 



A note in regard to the estimation of fat by the modified Rose-Gottlieb 

 method, C. Huyge {Rev. Gin. Lait, 8 {1910) No. U, pp. 326-328, fig. i).— The 

 author found the Eichloft" and Grimmer siphon method (E. S. R., 23, p. 217) 

 to furnish good results. 



Fat estimation in milk, W. Faheion (Chem. Ztg., 34 {1910), No. 73, pp. 648y 

 649). — A claim of priority in regard to the Rose-Gottlieb method as modified by 

 Eichloff and Grimmer (E. S. R., 23, p. 217), with some additional recommenda- 

 tions. 



A further examination of the " neu-sal " method of Wendler for the 

 determination of fat in milk, E. F. Notbohn and J. Angekhausen (Ztschr. 

 Vntersuch. Nahr. u. GenussmtL, 20 (1910), No. 8, pp. 495-498; ads. in Chem. 

 Ahs., 5 (1911), No. 3, pp. 535, 536).— The reagent used in the method (E. S. R., 

 23, p. 614) consists of a mixture of the salts of salicylic and citric acids colored 



