EFFECTS OF ALCOHOL ON INFUSORIA. 95 



(fi) Stylonychia. 



On November 6, 1907, a second culture of Stylonychia (culture 

 r) was started line by line from culture I in exactly the same 

 manner as Paramecunn T was started from Parmnccium I. The 

 treatment which followed was identically the same as that already 

 described for the Paramecium experiments — culture T being 

 subjected to one part of alcohol to 2,500 parts of culture medium. 

 The effect of this treatment is shown in Fig. 3, beginning at 

 period 4. It will be noted that the division rate of the alcohol 

 treated series (•-•-. line) was very much more rapid than that of 

 the control (continuous line) during the first five five-day periods 

 of the experiment. It fell slightly below the control during 

 the next period, but during the following two periods of the 

 experiment it was again far more rapid than the control. Thus 

 we find in the case of Stylonychia that the treatment with alcohol 

 of the strength employed produced, as in the Paramecium (culture 

 P) experiment, stimulation for about the first month of the work. 

 The plotted curve shows also that from this point on the rate of 

 division of this series fluctuated above and below the control and 

 at the present period it is again exceeding that of the control 

 culture. 



On November 26, 1907, culture V" was started from culture I, 

 and was thenceforth treated with double the amount of alcohol 

 (one drop of 2/500 alcohol and four drops of hay infusion) to 

 which the parent culture was subjected. The result, again simi- 

 lar to that of the corresponding Paramcciiivi culture, shows an 

 increased division rate for several five-day periods of the culture 

 subjected to the increased amount of alcohol. (Cf. Fig. 3, period 



8 through 14, line.) Again the stimulating effect was not 



continuous, but instead, as in the previously described experiments, 

 the division rate of the alcohol-treated line finally fell below that 

 of the control and remained below until the experiment was dis- 

 continued on December 31, 1907 ; though a new culture isolated 

 from this culture and stimulated with double the amount of 

 alcohol showed an increased division rate at first, and later de- 

 creased division rate as compared with that of the control culture. 



