CONTENTS. 



I. I n t r o d 11 c t a r y Remarks 17 



II. Historical Review 19 



III. Culture Media 34 



1) S u p p o r t i n g apparatus 34 



2) Growth promoting substances 41 



3j P li y s i c o - cli e m i c a 1 study of the nature 



of the g r o \\- t h promoting substances . . . 44 



Evaporation. Precipitation. Carbondioxyde precipita- 

 tion of the globulins. Alcohol precipitation. Autolysis 

 and hydrolysis of the proteins. Quantitative determina- 

 tion of amino acids in hydrolyzed embryonic tissue 

 juice and chicken plasma. The fatty components of the 

 growth promoting substances. Adsorption experiments. 

 Disssociation of adsorbed substances. Summary. 

 Growth of fibroblasts and hydrogen ion concen- 

 tration of the medium. 



IV. Tissue cultivation technique 80 



1) T e c h n i q u e applied here 80 



Prparation of the plasma. Preparation of the embry- 

 onic tissue juice. Making the cultures. Lists of instru- 

 ments. Preparation of the salt solutions. Preparation 

 of fibrinogen. The rate of growth and its estimation. 

 Selection of the tissues. Preparation of the tissue. Pre- 

 paration of the media. I^reparation of the cultures. 

 Preparation of the media. Preparation of the cultures. 

 Measurement. Cultures of tissues in a state of un- 

 interrupted life. The new method of Carre I's. The 

 methods of tissue survival ad modum Lewis 

 and Lewi s. The L o c k e -L e w i s's "culture me- 

 dium". Micromanipulation. Microphotography and mi- 

 crocinematography. 



3019S 



