12 



21 P r c jj a r a t i () ii of t i s s u c cult u r c s f o r in i- 



c r () s (• (I p i (■ a I ]> II 1" |) o s c s 97 



TIk' vilal slaiiiiiij^ Siini)li- staiiiiii<^ mclliods lloma- 

 loxylin. Melhylciu' blue. A/iir-l)liu- Stclioning llic 

 ciilliiri's. (lold i)r('i)arali()n of [issue cullurcs. V'wa- 

 tioii and slalning mclliods ol L i' \v i s. 



V. Pure strains of tissue cells 103 



1 (. (11 era I 103 



Melhods of ohlaiiiiiii; purr cultures. I'lie anatomical 

 nu'lhod. Ilie physiological elective inelliod 



2 l^M hrohl as ts 10.') 



3) E])i t hel ial eel Is 107 



Technique for Ihe preparation of the tissue. Tech- 

 nitjue for the preparation of the cultures. Charac- 

 teristics of Ihe ei)ilheliiini in cultures. Mixed cul- 

 tures of epithelium and fibroblasts. Avoid contami- 

 nation of the epithelial cultures witli fibroblasts. 

 Dedifferenliation. The behavior of the epithelial 

 cells and the fibroblasts to the stroma. The method 

 of D r e w for isolation of pure epithelium in vitro. 

 The cultivation of a neuro-epithelium and of a mu- 

 cous secreting epithelium from the intestine. 



4) Cartilage eel I s 129 



The preparation of the tissue. Technique of cultiva- 

 tion. Characteristics. Transformation of the cartilage 

 cells in cultures. 



5) Ly m ph o cy t es 134 



Preparation of the leucocytes for the cultivation. 

 Characteristics of the growth of lymphocytes in vitro. 

 Transformation of the large mononuclear lymphocytes 

 into fibroblasts. The responds of leucocytes to dif- 

 ferent amounts of embryonic tissue juice. 



VI. Tissue cultures as a physiological and 



a n a t o ni i c a I ni e4 li o d 142 



1) T h e humors of the body 142 



The properties of the body juices. The extracts of 

 various tissues. The influence of serum on fibroblasts. 

 The significance of the embryonic tissue juice to the 

 growth of tissue cells. The influence of serum from 



