220 



11 



I M () 1- p li () j4 I' II (• t i <• s 2()("> 



Adviiulagi's ;m(l (lis;i(l\;iiil:i<<rs of lissiic cultivali(jii 

 as a hislolof^ical iiicIIkkI I'lic slriiclurcs of Ihe coll. 

 Milochoiulria. Degeneration uiranules and vacuoles. 

 I'ignienl fornialion. Miloehoiidr-ia. granules and va- 

 cuoles in synipallielic ner\ (■ ril)ers. Degeneration oftis- 

 sue cells. I'ormalion oi' lat (iiant cenlrospheres. Giant 

 cells. Inlerrelhdar siihstniices ("onneclive tissue fibers. 

 I'he hyaline snhslance of Ihe cartilage. Classification 

 of llu' tissue cells culli\aled in vitro. Nerves. ImkIo- 

 derniai tissues. l-!ct(tdi'rmal tissues. Liver cells. 

 Thyroid gland cells. ISlood cells. Hone marrow cells. 

 Skclelal muscle. Smooth muscle. luidothelium. Car- 

 tilage. 



\' 11 . Tissue c 11 11 u r s :i s n ]) a I h o 1 o " i c a 1 ni e I h o d 220 



I .S t u (1 i e s o n i m m unit y a n d r e 1 a I c d p h e- 



n o m e n a , . . 



Ceneral Production of antibodies in vitro. Fi- 

 broblasts and immunity. Technique. The rate of 

 growth of fil)roblasts in media containing small 

 amount of a foreign protein. The respond of im- 

 munized and non-immunized fibroblasts to a large 

 dose of a foreign protein. Anaphylactic respond of 

 immunized fibroblasts? The calculation of the degree 

 of immunization (production of antibodies?). The 

 relation between concentration of antigen and the 

 degree of immunization. Anaphylaxia. Precipitins. 



2) Tissue culture as a bacteriological 

 method 237 



Effect of bacteriotoxins on living tissue cells in 

 vitro. Interaction of bacteria and living tissue 

 cells. The cultivation of various kinds of filti'able 

 virus. The cultivation of the virus developing the 

 R o u s chicken sarcoma. Tubercle bacilli and tissue 

 cells in vitro. 



3) Specificity of the tissue cells 238 



(^ytotoxins. Ceneral. Cytotoxins of malignant cells. 



4) T u m o r s 212 



General Characteristics of tumor cells cultivated 

 in vitro. Technique used by different investiga- 



