IS 



Till' ofrccl ol' the ^lohiilins .iiid iilhiiiiiiiis ohtnincd !)>• 

 prec'ipihilion witli CO.. |)i'()\f(l to he siiqhily more acliNC 

 than tile «^l()l)ulins ohlaincd l)\ prccipitalion wilii XH, .SO,. 

 I-'i'om llu' tahic 1 such an cxpcrinu'iil can he seen. 



To the conlrol culliires were added the ()ri<,niial untrealed 

 exlract. The total nitrogen was determined of the original 

 extract and of each of the two fractions containing the glo- 

 bulins and alhiiinins. (Table 2.) 



Table 2. 



Fraction 



Per cent 

 Total nitrogen. 



Original extract 0,126 



Globulin 0,047 



Albumin 0,062 



4. Alcohol precipilation. 



Several other methods were used for precipilating Ihe 

 different proteins, but without any remarkable i-esulls. The 

 method which gave the best results must be mentioned. 



To the embryonic tissue juice was added !)."> pei- eenl al- 

 cohol until a precipitate appeared which was (piiekly removed 

 by cenlrifuging, shaken with ether in order to I'emove the 

 alcohol and resolved in Ringer solution. II is important 

 to separate the precipitate quickly from the bulk. If not. 

 the proteins will denaturate and be difficult to bring in 



