:>() 



sufficicMil lo obtain a i)ivci])ilal(v Tliis was removed (juickly 

 by centiMfuginfT aiul washed Iwice with ellier and dissolved 

 ill .") c (• ol" H i n j« e r solulion. The rale of ^rowlii ol' libro- 

 blasls 1^10 years old strain "' was delermiiied lor the dif- 

 ferent fractions added to the culture medium As controls 

 served the orij^inal extract. 



From table 'A it can be seen, that the hJLfher the acidity 

 is, the lower is the alcohol nund)ei-. It can also be seen, 

 that the transparency of the redissolved alcohol precipitate 

 is highest at Ph. 6,8, at which reaction the precipitate was 

 easiest to bring lo solution. The effect of the different 

 protein fractions on the rate of growth is such, that it in- 

 creases markedly with the increasing alcohol number and 

 decreasing hydrogen ion concentration up to a certain point, 

 viz: to the normal reaction of the tissue juice at Ph. 0.8. 



Table 3. 



No. 



C. C. Pti.ofSc.c. 

 Embryonic Buffer 

 Tissue solution 

 Juice. added. 



nu s ^^■ ai u i Grade of 



Pti. of Mix- Alcotiol ™ , 



ture of Tissue Number ^ ^ 



Juice andBuf- in 



fer solution. C. C. 



4,0 

 4,6 



5,2 

 5,8 

 6,6 

 7,0 



7,8 

 8,4 

 9,0 



5.6 

 5,8 

 6,0 

 6,2 

 6,8 

 6,8 

 7,2 

 7,3 

 7,5 



3,5 



5,5 



6,0 



7,5 



9,5 



11,0 



11,5 



14,0 



16,0 



of 



Re-Dissolved 



Precipitate. 



XXXX 



XXXX 



XXX 



XXX 



XX 



X 



XXXX 



XXXX 



XXXX 



Relative 

 Increase 

 of Growth 

 of Fibro- 

 blasts. 



0,07 



0,8 



1,3 



1,8 



0,9 



2.4 



0,6 



0,8 



0,03 



X =- transparent, clear. 

 XXXX = higlily intransparent. 



This experiment does not seem, I believe, to indicate, that 

 we by this method get precipitation of different proteins. If 

 this should have been the case, we would not have got such 

 great differences in the alcohol number and would have ob- 



