53 



on the tissue cells, were willioiit success, more indirect 

 methods were selected with tlie purpose of studying (he 

 nature of this or tlu'se substances. A comparative quanti- 

 tative analysis was tlu-refore niiuh' of the amino acids in 

 the hydrolysed embryonic tissue juice and the plasma from 

 adult chickens, the latter containing none or very little 

 of the growth promoting substances. It was supposed that 

 some of the important amino acids were lacking or sparcely 

 represented in the plasma or tissue juice. The results of 

 these experiments were not very striking. In some of the 

 analyses of the extract, lysine and histidine were found not 

 to be present. In the plasma from the adult chickens all 

 the amino acids sought for w^ere there. It w^ould be rather 

 a interesting fact if the absence of these amino acids was' 

 constant in the hydrolysed proteins of the embryonic tissue 

 juice. 



Experiments No.s 85—87. 

 Table 4. 

 Quantitative .'determination of amino acids in tiydrolyzed ; embryonic 

 tissue juice and chicken plasma, a. m. Van Slyl<e. 



gram m 



100 cc. of 



tissue juice 



gram m 



100 cc. of 



ctiicken plasma 



Pricipitated 

 by ptiosptio- 

 tungstic acid. 

 Basic amino 

 acids. 



Total N. 

 Amid N. 

 Melanin N. 

 Total N. of the bases 

 Nonamino\ Arginin 

 Nitrogen / Histidine 

 Amino \ Lysine 

 Nitrogen I Cystine 



Total N. of the acid 

 amino acids 

 1) Amino N. of the acid 



amino acids 

 ■-) Non amino N. of the 

 acid groups 



0,108 



1) Contain 

 rine. Leucine, 



glutaminic acid, aspartic acid. Tyrosine, Phenylalanine, Se- 

 Isoleucine, Valine, Alanine, Glycocoll. 

 2) Proline, Oxyproline, Tryptophane. 



