62 



tratioii nindo by Carrol*' speaks vorv nuicli in favour of 

 this assuni|)lion. 



The tal)li' 7 iH'prcsciils an cxpcrinicnl of adsorption, lol- 

 lowc'd by [he dissociation l)y moans of diri'orent methods. 

 The adsorption and dissociation were all the time control- 

 led by nitrogen delenninalions of the various fractions. 



Table 7. 

 Adsorption and dissociation experiment. 



K,„ Grams of cc. of Pti. 5,6 cc. ^^^ ^^"* ^ 



^^°' blood coal. fresh extract. of buffersol. '" supernal. 



fluid. 



TIk" supernatant i'liiids were then drawn off and the 

 dissociating fluids were added to the blood coal and shaken. 

 The different tubes were treated in the following ways. 



1;3 c. c. of destilled water; two hours at 53 C; 15 hours in 



refrigerator. 

 2)3 c. c. of destill. w^ater; two hours at 39 °C.; 15 hours in 



refrigerator. 

 3)3 c. c. of 10 'Vi> saccharose sol. at 39 "C. for two hours; 



dialyzation and the volume adjusted. 

 4)3c. c. of Ringer solut.; two hours at 39 ^C; 15 hours in 



refrigerator. 

 5)3c. c. of Ringer solut. containing 0,2 c. c. of a 1 <'o solution 



of sodium olcate; two hours at 39"^ C; 15 hours in refri- 



gei'ator. 

 6)Two hours at 39 ' C. and 15 hours in the refrigerator. 



*) Unpublished experiments. 



