1)3 



The nitrogen was then tlelermined in the blood coal and 

 in the supernatant fluid separately after the dissociation 

 has taken place. The absolute increases of growth of fibro- 

 blasts were determined in Ihe vai'ioiis fractions. TTabie 8). 



Table 8. 



The undiluted original extract for these experiments con- 

 tained 0,2 1 o/o total nitrogen — and the absolute 

 increase of growth was found to be 12.7. 



When diluted with equal its volume of buffer solution Ph. 

 5,0, the total nitrogen was found to be 0,105 — and 

 the absolute increase w^as 6,2. 



The supernatant fluid from the adsorption experiments 

 showed a positive Heller, M i 1 1 o n and Biuret reac- 

 tion, but the dissociated substances did not give these reac- 

 tions. The following experiment illustrates this Table 9). 



Table 9. 



These adsorption experiments will probabh' pave the 

 way for the Investigation of such complex substances. The 

 interpretation of the experiments is of course difficult be- 



