72 



anioiml of cml)ry()iiic lissiu- juice oi" |)l;ism;i would j^ivc 

 the hy<li-()j,U'U ion couccnlrMliou (Icsircd 



Oi" Munllu'i' luctliod WMs lliis. Iii;il S o r c n s c n s slauchird 

 |)hos])ii:\t(' solutions, stcrili/cd in pressure hollies. weiX' ad- 

 ded lo llu cMuhryc^iiic lissue juice in e(|ual volumes lo ohlain 

 dilTercnl hydrogen ion concen I rations ol' the media. ll 



was ol)served that mixlure ol end)rvonic lissue Juice and 

 Ihese slandard pliosphale solutions had no inhihilin<f elTecl 



pH 

 9 



5 6^89 



pH 



Fig. 3. 



The abscissae indicate tlie pH obtained, and the ordinates the pH of the 



buffer solution added. Curve 1 represents the pH of Ringer solution, and 



Curve 2 the pH of embryonic tissue juice, to both of which are added the 



different standardized buffers as indicated by the ordinates. 



on the growth ol' I'ihrohlasls except when the liydrogen 

 ion concenlralion was such Ihal this alone caused an inhi- 

 hiling inl'luence on llie growth ot the tissue. A known 

 hydrogen ion concentration was ohtained hy the I'ollowing 

 lechni([ue. I^reliminary experimeiils were made in order 

 lo find oul the I'h of a mixlure of emhrNonic lissue juice 



