99 



The scheme for the resL of the preparation is the follow- 

 ing when hematoxyhn, foi' instance, is used for slauiing: 



(1) After fixation, washing in running water 3 hours at least. 



(2) Distillec water one hour. 



(3) Hematoxylin (M i n k's and Paul M e y e r's modification) 

 50—60 drops in 50 c. c. of water 10 to 12 hours. 



(4) Washing in water half an hour. 



(5) Five minutes in 50 o/o alcohol. 



(6) Five minutes in 75 o/o alcohol. 



(7) Five minutes in 95 o/o alcohol. 



(8) Ten minutes in a mixture of 95 o/o acetone and 5 o/o xylol. 



(9) Ten minutes in a mixture of 70 o/o acetone and 30 o/o xylol. 



(10) Ten minutes in a mixture of 30 o/o acetone and 70 o/o xylol. 



(11) Fifteen minutes in xylol (I) 



(12) Fifteen minutes in xylol (II). 



(13) Embedding in b a 1 s a m u m canadensis. 



Another method must be mentioned, which gives excellent 

 results because of the fact that the clot is decolorized 

 in the acetone-xylol mixture and allows of a beautiful dif 

 ferentiation between the stained cells and the colourless 

 plasma-clot. Besides that, the following method does not 

 give any precipitation in the culture, what the hematoxylin 

 often gives. The dyes used here are methylene- or azur- 

 blue. 



(1) Fix in 2 ob Formalin-Ringer's solution one hour. 



(2) Running water 3 hours. 



(3) Five minutes in distilled water. 



(4) Flood the coverglass with Loeffler's alkaline methylene 

 blue and hold it over a pilot light until vapor appears. 

 Remove from flame and allow stain to remain on speci- 

 men for about fifteen minutes; then pour off the stain. 



(5) Wash with water from a Pasteur pipette. 



(6) Dehydrate in alcohol: 50 o/o, 75 o/o, 95 o/o, one minute each. 



(7) Acetone-xylol mixtures (5 o/o, 30 o/o, 70 o/o xylol) two 

 minutes each. 



