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rislu's liMs. MS is known, often a pars (■(irlildf/o srlcrdc imd 

 soiiu'linu's I'viMi ;i pars ossca. 



J'hc cxpcriiiu'iil \v;is iiiKlcrlakcn in llie loliowiiiy; way: 

 On the excised eye l)nll), lIic dai'k pignieiiled in e m 1) r a ii a 

 iiiclilans was pnslu-d aside with a small knile and l)y 

 means of an iris I'orccps Ihc lliin semi Iransparenl membrane, 

 whicli is the cartilage, was taken out. The eyes used, were 

 taken I'roni ehieken-embi-yos about 15 18 days old. The 

 manipulation was not so easy as it apj)eared Irom tlie 

 beginning. xMany pieces of cartilage proved later to contain 

 elements of connective tissue and the cultures were discarded. 



The technique for the cultivation was briefly this. The 

 fragments were placed on the free surface of the coagulated 

 plasma-juice mixture and covered with a little Ringer's 

 solution or tissue juice. The composition of the culture 

 medium was the same as that used for fil)r()l)lasls and 

 epithelium. 



The cartilage did not grow at all when it was emlK'dded 

 in the plasma clot. The fragment was loosened and fell 

 to pieces and proved to be extremely difficult to transfer 

 to a fresh culture medium. The appearance of such a 

 culture of cartilage cells which was cultivated for some 

 time embedded in the clot looked almost like a culture of 

 yeast. There was no cohesion of the individual cells, which 

 were round and extremely small. By examination with high 

 magnification, the cells had much the same appearance 

 as lym])hocytes. They were of the size of small lymi)hocytes 

 with a very sparse cytoplasm and a nucleus which look 

 the stain very intensively (Fig. 22). The stroma disappeared 

 after quite a short time of cultivation and the tissue fell 

 apart. In making stained specimens after varying intervals 

 of incubation, the loosening of the tissue could be followed 

 quite well step by step (Fig. 22). The hyaline substance 

 was loosened and finally disappeared. The cells migrated 

 out or became free of the amorphous substance, but did 

 not produce any new^ and died. 



