134 



1)0 lollowc'd sli'p by slcp. Here we have a tissue, whieli in 

 the orj^anisin, iiiitler ceiiain coiulilions |)ro(liiees a peeiiliar 

 stroma which, when broiij^'iil lo a eiilliire medium under 

 cerlain ex]ierimenlal coiKhlious (lisa])pears. Another interest- 

 ing Tael is Ihal (lie lissue is una])le lo '^row when il 

 is euibedded in the clot; only oji the surl'ace the cells are 

 able lo spread out. 'JMiere is no doubl thai Ihis phenouienon 

 is due lo tlie (lilTereul |)livsical coudilioiis which exist in 

 the bulk and on tlie surl'act' ol' the clol. 



/ 



/ 



I 



^ / 



• ••<■ r.^ •• i 







,•« Oy 



' - • • .e«« 



'^ — " ./ ••,•»•,*© o o »'».• "" 7 



Fig. 24. 



The segregation apparatus of R e n a u t, vitally stained with neutral red. 



The cultures are 6 Weeks old; 24 hours incubation. 



From The Journ. of Exp. Med. 1922, XXXVl, 379. 



LARGE MOXOXUCLEAR LEUCOCYTES. 

 In 1921 Carrel and Ebeling"*5) succeeded in obtain- 

 ing a pure culture of large mononuclear leucocytes. The 

 method used here for obtaining the pure cultures is the 

 physiological elective. The method is briefly the following: 

 The white blood cori)uscles are obtained by cenlrifuging at 

 high speed the freshly taken blood from an adult chicken. 



