18H 



Aric'i" the rcinoN.il of Ihc phisiiui. ;i lew drops ol' dilulcd 

 cmhryoiiic lissiic juice is |)l;tc('(l on IJic siirr;icc of Mic conl 

 oT h'lK'ocvlcs. .\l)oiil riflccn mimilcs hilcr. Ihc I'oa^iilimi 

 conlaininij Hu- while ci'lls can he renioNcd and phiccd in a 

 walch uiass willi a small aniounl ol' |{inii;ers solution. 

 Nearly all (he red cells can now he washed awa\ and small 

 fragmonl.s of (his coa<«ulum conlaininu; Ihe while cells can 

 he ti'misTerred lo llie cullui'e medium The medimn 



Fig. 26. 

 Experiment 1634. Mitotic figures in cartilage cells. Stained with azure blue, 



X 1,000. 

 From The Journ. of Exp. Med. 1922, XXXVl, 379. 



used foi" cullivalion consisted either ot two xolumes ol' 

 plasma and one voliune ol" chicken emhryo juice, or ol' one 

 volume oi' plasma and one xolunie ol' chicken end)ryo 

 juice, and two volumes ol' lyrode solution, (irt^at care 

 must he taken lo avoid conlaminalion of llie cnltui'cs with 

 other cells from tlie tissue juice. Therefoi'e. the end)ry.)nic 

 juice was diluted with Tyrode solution and cenlrifuu,ed foi' 

 a long lime at high speed. 



