137 



Small fragmenls of the coaftulimi liom llic hlood. con 

 laining the while corpuscles were emhedded in the plasma, 

 (issue juice was added and coaguhdion then lakes place. 

 II is inipoi'lanl lo know Dial il' lliei-e are (oo lew leuco- 

 cytes in Ihe cullui-e or if there are loo many, they die. 

 After forty-eighl hours incuhalion. or sometimes even longer. 

 1—5 days, the original fragment was cut out. washed in 

 Ringers solution and placed in a I'resli culture medium. 

 Here it is very important to cut the cultui-e out ot the 









-^Ms^'-'^-^'^^-i 



Fig. 27. 

 24 hour culture of leucocytes taken from the blood. In the upper left corner 



is seen part of the original mass of cells, x 62. 



From Carrel and Ebeling: Large monunuclear leucocytes. The Journ. 



of Exp. Med. 1922, XXXVI, 365. 



clot with clean cuts. If IIk^ edges ai'e crushed or folded, no 

 migration takes place. 



If the cultures were made in the projjcr way. the cells 

 could he seen to migrate into the medium after twenty- 

 four hours. The outer zone was formed of small amehoid 

 cells, which in stained preparations |)roved to he pol}- 

 morphonuclear leucocytes (Fig. 27 . The inner zone con- 



