138 



sisted of Inrj^'tT :niicl)()i(l cells, soim- will) larjfc relicuhii- 

 pseu(l()|)()(ls and olhcrs with siiinll liiiform or ]ol)ar pst'ii- 

 dopods. Allor a few passaj^'cs llic poiyinorijhomiclcar leu- 

 cocytes disappeai-cd and a iilllc lalcr also Ilic sinali lyni- 

 phocvtos. The cells whieh remained and conlinued lo mul- 

 liply were Ihe lar<f(> niononnelear cells. The ^'eneral appear- 

 ance ol" the culture was vei-y different from a culture of 

 connective tissue cells. The c t- 1 1 s had no li- n d e n c y 



Fig. 28. 

 Colonies of leucocytes in a Gabritschewski dish, showing their lacl< of 



coalescence. 



From Carrel and Ebeling: Large mononuclear leucocytes. The Journ. 



of Exp. Med. 1922, XXXVI, 365. 



to form a tissue, hut remained isolated. When colonies 

 of leucocj'tes were grown together in a G a b r i t s c h e w- 

 s k i dish, the colonies showed no tendenc}' to unite as 

 colonies of fibroblasts or epithehum always do (Fig. 28). 



The large mononuclear cells which migrated into the 

 new medium were elongated or branched. The anterior end 

 of the cells showed usually very active pseudopods. When 

 the cells were stained the nucleus become dark ami 



