lo9 



vourable culUirc medium. It was found that if the Iransplan- 

 lod piece consisted of a few scattered cells no growth look 

 place, and the cells took on the aspects of degeneration at a 

 time when the control culture seemed to he in perfect con- 

 dition. By contrast, when the small clumps of cells had 

 been taken from the central portion of the culture where 

 it was dense, growth was extensive. The tissue used in these 

 experiments was derived from a 4 month old strain of fibro- 



B 



A 



rWband 



Wound 



Fig. 32. 



A, outline drawing of the culture shown in Fig. 33 after incision. The limits 

 of the mother culture, the separated culture, and the wound are to be seen. 



B, outline drawing of the same culture after 24 hours incubation. The mo- 

 ther culture has almost doubled in size since the wound was made but the 



separated fragment has ceased to grow. 

 From The Journ. of Exp. Med. 1923, XXXVlll, 671. 



blasts, isolated and cultivated in the Insliliile of General 

 Palliology, Copenhagen. The small fragments were obtained 

 by clean cuts with a sharp cataract knife. After a little prac- 

 tice fragments could be cut so small that they could only 

 just be distinguished with the naked eye. 



Here it might be objected that the poor growth of the 

 fragment containing onlv scattered cells was a result of the 



