218 



cells Mild tissues was liiuilly iikkIc hy Lewis and L e- 

 w i s :5^3). 



I'" c to derm I'roni skin was sliidicd l)y (larrel and 

 U II I- row s ■' . L a in he r I -"'i. T h I e n h ii I li ''" H u 111 "■';. 

 Holmes -'•^). () |i ]) c 1 '•'). M a I s II 111 o I o ""^ ; IVom tlie 

 amnion by Lewis and Lew is 'j^j iVom Ihe relina by 

 rii I e n h u I li '■'' , L ii n a '-• >. Smith ^''-), V i s c li e r 2"0). 

 and from Ihe iris by F i s e h e r "*-), E b e 1 i n g and V i s e h e r 

 167), Ebeling les). 



Liver cells also form membranes similar (o the enioderm 

 and were described by Lynch •''!•"). '['lie renal epithelium 

 trom the lubules also grows out in the form of membranes 

 or sheets (Lewis and L e w i s) •'■'^^;. and sometimes as 

 tubules ((^hampy) i^O); n^. thyroid gland cells grow out 

 either as tubules or membranes, ((barrel and Burrows; 

 ^*). Recently Ebeling*) claimed to have cultivated the 

 thyroid gland cells pure for a long period of lime, which 

 were |)roducing colloid substance all tlie lime in vitro. 



Blood cells and wandering cells from the s|)leeii have 

 been studied by Carrel and Ebeling. '"'i I'' o o t -i*') 

 M a X i m o f f i^"); bone marrow cells by C a r r e I and 

 Burrows ^4) Erdmann ^'^-): Lymph nodes by Lewis 

 and W e b s t e r •^•'■^;; Thymus by P a p p e n h e i m e r *^^). 



Skeletal muscle has been beautifully described by 

 M. R. Lewis ■^^'^^ and Lewis •^•''^). They sup[)ose that the 

 cross striated muscle fibres grow belter in Locke's solution 

 than in any other media, since among the numerous con- 

 tributions lo tissue cullure. so lillle has been said about 

 cross striated muscle by other observers who have used 

 plasma media. Lewis and Lewis obtained such abundant 

 outgrowth of muscle in their cultures. They describe that 

 the muscle fibres were able to contract rhythmically for 

 long periods of time. It was observed that there is a 

 marked tendency for anastomosis and fusion of muscle 



*) Ebeling, A. H. Personal communication. 



