221 



and the serum Ironi the control cultures did not possess 

 any activity. 



It was observed liiat the guinea pig leucocytes took up 

 the goat red blood corpusles by phagocytosis. 



The nature of the hemolysins was also investigated. The 

 fluid hemolysed goal red blood cells without the addition 

 of complement. Alter having been heated to 5G C. for half an 

 hour it lost its hemolytic power. Reactivation could be 

 established by adding complement. It was also found that am- 

 boceptor in the fluid from the cultures, could be adsorl)ed l)y 

 goat red blood corpuscles in 4 hours at O^'C, According to 

 these properties observed for the hemolysins produced in 

 vitro, C a r r e 1 and I n g e b r i g t s e n ^o) concluded that tis- 

 sues living outside of the organism react against an antigen 

 by the production of an antibody. 



Later R e i t e r ^^^) has worked with the production of 

 antibodies partly in vitro by using the modified technique 

 of Liidke^"^'); the technique used by Reiter cannot 

 be called tissue cultivation at all. He worked witli entire 

 organs from animals which previously had been injected 

 with bacteria cultures as an antigen. Reiter observed that 

 the production of antibodies began directlj^ after the injection 

 of the antigen, and he confirms the assumption of W a s- 

 s e r m a n n. P f e i f f e r and D e u t s c h that the antibodies 

 are essentially a product of the hematopoetic organs. 



The effect of specific hemolysins of living tissues in 

 cultures has been tried by H a d d a and Rosenthal -i*). 

 Their intention with the experiments was to investigate 

 the effect of cytotoxins on tissue cells. This paper will 

 therefore be discussed when the cytotoxic immunity and 

 cj'totoxins in general will be encountered. 



Lately I have ^-'^^ ^^^) studied the action of a small amount 

 of an antigen on the rate of proliferation of fibroblasts in 

 vitro. My intention with the experiments was to learn 

 whether it was possible to have a strain of fibroblasts 

 immunized against a foreign protein. 



