221 



scries [\\v suhciilliirc.-. were placed in media c()nlainin<^ 50 o/o 

 anlij»cii ascitic lliiid This aiiiouiit exeiied a marked in- 

 hil)ilinj4 action on Ihe rale of },fro\\ih ol' a normal culture 

 of lihrohlasls. The rale ol" <fro\vlh ol' the contiol cullure 

 which had nol heen ex])osed to ascitic lluid decrea.sed 

 markedly oi" died, t'i^. 17. Al i)assages Xos. 7. 10. 12. 11 

 and 17, hall" the tissue fragmenl was cullivaled in a medium 

 conlainint^ about 50 per cent ol' ascitic fluid, while the 



26 

 24 



p; 6 



12 3 4 5 6 7 



8 9 10 U 12 13 H 15 16 17 18 19 20 



Fig. 46. 

 In all the figures the ordinates represent the relative increase in square 

 centimeters and the abscissae the number of passages at 48 hour intervals. 

 The rate of growth of tvi'o cultures of fibroblasts, the control (dotted line) 

 cultivated in the usual homogenic culture medium, the other (solid line) 

 in the same medium containing 7 per cent ascitic fluid. 



other hair was used lor the continuation of the strain. The 

 dots A, B. C, 1) and E fig. 47) represent the rate of growth 

 of these subcultures during 48 hours. It can be seen that 

 ascitic fluid in a concentration of 50 per cent has a marked 

 inhibiting power on the rate of growth of a normal culture 



