236 



I'lu' |)lu'n<)iiu'n()n ol" lUKiphylnxiM has iiol been sludicd by 

 means ol' lissiic ciiltix alion. Allcmpls liaxc Ix-cii made U) 

 invcsli,i<alc thr cellular aclion uikIcm- aiia|)hylaclic roaclions 

 by means oi' isolated or<,nins. i. c. iilenis, lungs, liver sus- 

 pended in Ringers fluid, (W e i 1 '••') ■''•^), M a n w a r i n g 

 and ( ", r o \v ^''**) ^^^). 'I'he tissue eultixalion lechni(pu> will 



Immunized strain 

 B. B, 



Non-immunized atP<ain 



Non-immuniied strain 



Fig. 56. 

 A is a culture witti a small amount of antigen, .li and .Ao stiow ttie same 

 culture divided in tialf. Ai is carried on in a medium containing a small 

 amount of antigen; A-j (subculture) is transferred to a medium containing 

 ttie antigen in a higti concentration. B is the control culture. Bi is carried 

 on as B, the control culture, and B^ is transferred to a medium containing 

 the antigen in a high concentration. The quotient of Ao divided by B^ 

 expresses the degree of immunization of Ai. 



probably also here be of excellent help for the investigation 

 of anaphylaxia. The stimulation of the rale of growth, as 1 

 found it for •immunized' fibroblasts when exposed to a 



