241 



med iurlher that llie immune plasma from I lie rabbit against 

 chicken bone marrow cells, also had a marked cytotoxic 

 effect on the rabbit bone marrow cells. 



All these interesting investigations oufflit to be again taken 

 up since we are now able to master the technique of I issue 

 cultivation and since now we know a good deal more of the 

 action of heterologous culture media on the tissue cells. 



It is difficult to find out what the general opinion is 

 as to the existence of an organ specificity. Many investigators 

 concluded from their experiments, especially from a study 

 of the lesions found in animals, injected by "specific 

 organ immune substance', that such a specificity 

 can be demonstrated. According to Lambert 295). L ii d k e 

 and S c h ii 1 1 e r ^^^\ they have described the production of 

 a nephritis after the injection of the serum of rabbits im- 

 munized with dbg kidney. Pearce ^^t) explains the various 

 lesions following the injections of cytotoxic sera as a direct 

 toxic action on certain parenchymatous cells, of the hem- 

 agglutinative and hemolytic properties of the serum, devel- 

 oping thrombosis with all its consequences. He does not 

 believe at all in specific somatogenic cytotoxins. 



Lambert 295) claims in his paper on this subject thai 

 the problems can be solved from the methods of tissue cul- 

 tivation, because here the active living cells may be ex- 

 posed to the action of the immune sera, and under ex- 

 perimental conditions, factors such as agglutination, throm- 

 bosis and hemorrhage can be eliminated. 



L a m b e r t 295) described the experiments he has under- 

 taken with rat sarcoma and rat embryo skin, experimenls 

 which fully confirm what has been found earlier by other 

 methods. 



Guinea-pigs were inoculated subcutaneously with 0.15 

 gram rat sarcoma, and others with a similar amount of 

 embryo skin. After the termination of the immunization, 

 the animals were bled and the plasma obtained for the cul- 

 ture. Plasma from normal guinea-pigs were used as controls. 



16 



