217 



sarcoma in this nuMliuiii was tho same as descril){'cl carlici- 

 by Carrel and B ii r i' o \v s "'' . The colls arc mainly aclive 

 ameboid and wander far out in the plasmic medium. 

 Already after a few hours cultivation, a very extensive li- 

 quefaction occurs and the migrated ameboid cells can now 

 be seen contracted as spherical bodies, floating around in 

 a lake of liquefied plasma clot. 



To the cultures of sarcomatous tissue were now added 

 small bits of muscle, excised directly from normal adult 

 chickens, or taken from fragments of muscle which had 

 been stored in Ringer solution for a long time in the re- 

 frigerator. The small fragments of muscle, of about the 

 sizes of the explained tissue itself, were placed almost in 

 contact with the fragment of sarcoma in the culture medium. 



The muscular tissue is taken from the chickens, from 

 the muscles of the neck, when the chickens are bled to 

 obtain the plasma. The muscle-fragment is quickly placed 

 in a small jar with Ringer solution, and stored in the re- 

 frigerator. As often as it is to be used, it is taken out and cut 

 into small pieces. These are generally embedded first in 

 the usual culture medium by themselves and incubated for 

 about 2 — 4 days and thus tested this way for infection and 

 to see if any outgrowth from the muscles takes place. Not 

 a single cell has been observed to grow out from such a 

 piece of muscular tissue, stored in the ice box from 24 

 hours to one month. 



The muscle used for the experiments is always selected 

 to be of as pure muscular tissue as possible, witliout tendinre 

 or connective tissue. By storing the muscle-fragments for 

 about 14 days in the refrigerator, all the cells have died and 

 in any case are not able to grow in the cultures. 



After having tested the fragments of normal muscular 

 tissue in the way just described, they are cut out of the 

 plasma clot, washed in Ringer solution for about half 

 a minute and a single piece is placed together with the sar- 

 comatous tissue, side by side in the same culture. When 



