249 



actly as the original fragmonl of sarcomatous tissue, i. e. it 

 liquefies the culture medium in the same peculiar way 

 as did the original cxplanled tumor tissue hy producing a 

 very mucous secretion, and a corona of ameboid cells can 

 be seen migrating out from the muscle, representing the 

 same types of cells as found in the original piece. 



Fig. 57. 



Photo of a living culture of thie Rous's sarcoma. Debris and fragments of 



muscle's can be seen to the left, floating in the liquefied plasma. 



By and by the muscle gets thick and intransparent and 

 debris of decayed bundles can be seen floating around 

 together with the spherical cells in the liquefied plasma, 

 Fig. 57. Before the muscle fragment is entirely destroyed, a 

 new piece may be added or the old fragment divided up 

 and subcultured with the addition of fresh muscle. 



