263 



a mere conlact or cohesion between the cells, which so 

 often can be observed to take place between normal fibro- 

 blasts, for instance, bul a verital)le amalgamalion into one 

 cell. It has so far been impossible lo observe an uneven 

 distribution of Ihe granules ot Iwo united cells, which, 

 on the conlrary. would indicate a pseudo-amalgamation. 

 After such an amalgamation of two or several cells has 

 taken place, they generally separate shortly after into just 

 as many individuals as they were before united. 



Fig. 67. 



Type of the big ameboid cells ofjthe Rous's sarcome 



stained with methylene blue. 



This phenomenon of perfect amalgamation was observed 

 in all the cultures of malignant cells, including the various 

 sarcomata and the F 1 e xner-.To bl i n g rat carcinoma. It 

 has never been observed to occur in normal fibroblasts. 



Some few times, a very interesting j^henomenon was 

 observed namely, that two cells, belonging to the type of 

 the big ameboid cells, amalgamated perfectly, and shortly 

 after their separation, about one to two minutes, each cell 

 divided into two at the very same moment. The new cells 

 were much smaller than the mother cells. This was rather 

 a surprising fact, as it has never been observed in normal 

 tissue cells. The observations were always made with extreme 



