JEAN BRACHET 



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DL-3-phenylalanine-2- 14 C et de glycine-2- 14 C. Role des formes isomeriques de la 



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of the sea-urchin, Arbacia punctulata. J. cell. comp. Physiol. 6, 101-116. 

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isolated nuclei. J. gen. Physiol. 35, 559-578. 

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aspects of their glycolytic metabolism. J. gen. Physiol. 36, 181-200. 

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l'acide ribonucleique chez Acetabularia mediterranea. Arch. int. Physiol. 62, 154. 

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coeruleus x S. polymorphus. J. exp. <W., 124, 63-103. 

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d ! Amoeba proteus. Biochim. Biophys. Acta 9, 108-109. 

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1232 pp. 



Discussion 



Chairman: E. £euthen 



J. F. Danielli. Professor Brachet's results show that in enucleated fragments there is a 

 decline in RNA content and a decline in protein sythesis. These results are compatible 

 with the view that RNA is concerned in protein synthesis, or as I have suggested 

 elsewhere, (1953, Cytochemistry, John Wiley, New York), with the view that RNA 

 acts as a folding or trapping agent for protein. Is Professor Brachet aware of any 

 evidence incompatible with the second suggestion ? Dr. Yemm has already pointed 

 out that plant virus nucleoprotein does not exchange amino-acids once it is combined 

 with RNA. Another phenomenon which is explicable on this basis is the occurrence 

 of protamine in sperm; being a highly basic protein it combines with nucleic acids 

 and prevents protein synthesis, thus leading to sperm having little protein other 

 than nucleoprotein. 



102 



