JEAN BRACHET 



J. Bracket. The decrease in uptake of 32 P in non-nucleated halves is the earliest 

 chemical change so far discovered. The ATP content of non-nucleated halves does not 

 decrease in the presence of air for as long as ten days. As a matter of fact the non- 

 nucleated Amoeba, as well as the lethal frog's eggs, seem rather to have too high an 

 ATP content: this might mean that they are unable to utilize ATP. The marked 

 decrease in the ATP content of non-nucleated halves under anoxic conditions is 

 also a very early phenomenon, possibly simultaneous with the reduction in uptake 

 of 32 P. 



E. Hoff-Jorgensen : Microbiological methods, which are useful for the estimation of 

 coenzymes, might be used in the investigation of the effect of the nucleus on the 

 synthesis of coenzymes. 



104 



