18 



Frances Carter, Marion C. Woods and Miriam E. Simpson 



in which 10",, ICSH was added to the purified FSH during the 

 preparatory treatment. ICSH was then elTective as the ovulatory supplement 

 in a greater number of animals, and at lower dose levels, than when following 

 the FSH alone (Table 19). These results were confirmed with HCG (Table 20). 

 FSH was similarly evaluated as an ovulatory supplement at different dose 

 levels, following different preparatory treatments. FSH had previously been 

 shown to be effective as a supplement at the 8 RU level following follicular 

 development by 2 RU FSH + 8 RU ICSH. This held true for several FSH 

 preparations of increasing purity (Table 16). As shown in Table 21, 4 RU of 



Table 21. Graded Doses of FSH as the Ovulatory Supplement, after 

 Follicular Development by Different Treatments 



* Not counted; distended oviducts. 



purified FSH (MED 4 jug, 4% ICSH contamination) was still effective as a 

 supplement after preparation of follicles by 2 RU FSH + 8 RU ICSH. 

 However, 8 RU FSH was less effective as a supplement when the follicles 

 had been prepared by 4 RU of this FSH alone. The preparation used in 

 determining the standard conditions, containing 10% intrinsic ICSH, was 

 equally effective as an ovulatory supplement at 4 RU or at 8 RU, following 

 follicular development by 4 RU of this preparation. 



This apparent non-specificity of the gonadotropic supplementary stimulus 

 to ovulation leaves several questions unanswered. That time is not the factor 

 has been shown by failure of ovulation after the preparatory treatment alone, 

 even though the rats were autopsied simultaneously with those which had 

 received a supplementary injection in the interim. The occasional release of 



